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In continuation to our previous correspondence, the customer has tried these recommendations for blotting but with no success. She has also tried the antibody on Cos7, Hela and NSC34 cell extracts, with 2x phosphatase inhibitor mix and two different buffers.
She has not gotten yet a band for this antibody. She also checked pERK and got the expected band.
Thanks in advance for your reply.
Asked on Nov 09 2012
Thank you for your message and for providing this further information.
I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time they have spent on these experiments, it is disappointing this has not worked for the customer. I would like to offer a refund or credit note in compensation (providing the product has been purchased in the last 6 months). In order to arrange this, I would appreciate if you could confirm the order number and date of purchase?
Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.
Answered on Nov 09 2012