Key features and details
- Rabbit polyclonal to Stathmin 1 (phospho S25)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Stathmin 1 (phospho S25) antibody
See all Stathmin 1 primary antibodies
DescriptionRabbit polyclonal to Stathmin 1 (phospho S25)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Stathmin 1 (phospho S25). A phospho specific peptide corresponding to residues surrounding S25 of human Stathmin 1.
Database link: P16949
- Jurkat cell extract, treated with PMA; Human breast carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 17 kDa.|
FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
Sequence similaritiesBelongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain.
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
All lanes : Anti-Stathmin 1 (phospho S25) antibody (ab194752) at 1/500 dilution
Lane 1 : Jurkat cell extract, untreated
Lane 2 : Jurkat cell extract, treated with PMA
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Stathmin 1 with ab194752 at 1/50 dilution.
ab194752 has been referenced in 1 publication.
- Chen X et al. The Cdc2/Cdk1 inhibitor, purvalanol A, enhances the cytotoxic effects of taxol through Op18/stathmin in non-small cell lung cancer cells in vitro. Int J Mol Med 40:235-242 (2017). PubMed: 28534969