Overview

  • Product name

    Anti-Stathmin 1 (phospho S63) antibody
    See all Stathmin 1 primary antibodies
  • Description

    Rabbit polyclonal to Stathmin 1 (phospho S63)
  • Host species

    Rabbit
  • Specificity

    ab192648 detects endogenous levels of Stathmin 1 only when phosphorylated at serine 63.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Stathmin 1 (phospho S63) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. [Peptide sequence around phosphorylation site of Serine 63 (R-R-K-S(p)-H) ].
    Database link: P16949

  • Positive control

    • COS cell extract treated with nocodazole; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab192648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 17 kDa.

Target

  • Function

    Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
  • Tissue specificity

    Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
  • Sequence similarities

    Belongs to the stathmin family.
    Contains 1 SLD (stathmin-like) domain.
  • Post-translational
    modifications

    Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf215 antibody
    • Lag antibody
    • LAP 18 antibody
    • LAP18 antibody
    • Leukemia associated phosphoprotein p18 antibody
    • Leukemia-associated phosphoprotein p18 antibody
    • Metablastin antibody
    • Oncoprotein 18 antibody
    • OP 18 antibody
    • Op18 antibody
    • p18 antibody
    • p19 antibody
    • Phosphoprotein 19 antibody
    • Phosphoprotein p19 antibody
    • pp17 antibody
    • pp19 antibody
    • PR22 antibody
    • Pr22 protein antibody
    • Prosolin antibody
    • Protein Pr22 antibody
    • SMN antibody
    • Stathmin antibody
    • Stathmin1 antibody
    • STMN 1 antibody
    • Stmn1 antibody
    • STMN1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Stathmin 1 (phospho S63) antibody (ab192648) at 1/500 dilution

    Lane 1 : COS cell extract treated with nocodazole
    Lane 2 : COS cell extract treated with nocodazole with antigen-specific peptide

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Stathmin 1 (phospho S62) with ab192648 at 1/50 dilution (left image), or the same antibody preincubated with blocking peptide (right image).

References

ab192648 has not yet been referenced specifically in any publications.

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