Key features and details
- Rabbit polyclonal to Stathmin 1 (phospho S63)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Stathmin 1 (phospho S63) antibody
See all Stathmin 1 primary antibodies
DescriptionRabbit polyclonal to Stathmin 1 (phospho S63)
Specificityab192648 detects endogenous levels of Stathmin 1 only when phosphorylated at serine 63.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Stathmin 1 (phospho S63) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. [Peptide sequence around phosphorylation site of Serine 63 (R-R-K-S(p)-H) ].
Database link: P16949
- COS cell extract treated with nocodazole; Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192648 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibody was removed by chromatogramphy using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Predicted molecular weight: 17 kDa.|
FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
Sequence similaritiesBelongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain.
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
All lanes : Anti-Stathmin 1 (phospho S63) antibody (ab192648) at 1/500 dilution
Lane 1 : COS cell extract treated with nocodazole
Lane 2 : COS cell extract treated with nocodazole with antigen-specific peptide
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Stathmin 1 (phospho S62) with ab192648 at 1/50 dilution (left image), or the same antibody preincubated with blocking peptide (right image).
ab192648 has not yet been referenced specifically in any publications.