Product nameAnti-Stathmin 1 (phospho S63) antibody [EPR1574]
See all Stathmin 1 primary antibodies
DescriptionRabbit monoclonal [EPR1574] to Stathmin 1 (phospho S63)
Specificityab76583 only detects Stathmin 1 phosphorylated on Serine 62. The antibody immunogen shares 86% homology with Stathmin-2, therefore it is possible that the antibody will cross-react with Stathmin-2 when phosphorylated at serine 97. This has not been assessed experimentally.
Tested applicationsSuitable for: Dot blot, IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Human
Synthetic peptide within Human Stathmin 1. The exact sequence is proprietary.
- HeLa cell lysates treated with Calyculin A; human brain tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab76583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/2500 - 1/10000. Predicted molecular weight: 17 kDa.|
FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
Sequence similaritiesBelongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain.
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
All lanes : Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with nocodazole at 100 ng/mL for 18 hours. Whole cell lysates
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with nocodazole at 100 ng/mL for 18 hours. Whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 30 seconds
Blocking/Diluting buffer and concentration 2% BSA/TBST
ab76583, at a 1/250 dilution, staining Stathmin 1 in paraffin embedded human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) at 1/10000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Dot blot analysis of Stathmin 1 (phospho S63) phospho peptide (Lane 1) and Stathmin 1 non-phospho peptide (Lane 2) labeling Stathmin 1 (phospho S63) with ab76583 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Alkaline phosphatase treatment removes S63 phosphorylation. Samples treated with phosphatase were run alongside the normal lysate, and the phosph-S63 signal is not detected after phosphatase treatment, thus suggesting the signal is very specific to the phosphorylated S63.
Whole cell lysate prepared from a human colon cancer cell line was loaded at 20µg.ab76583 used at a 1/1000 dilution.
Secondary used was an HRP conjugated goat anti-rabbit polyclonal used at a 1/10000 dilution.
This product has been referenced in:
- Chen S et al. Introduction of exogenous wild-type p53 mediates the regulation of oncoprotein 18/stathmin signaling via nuclear factor-?B in non-small cell lung cancer NCI-H1299 cells. Oncol Rep 41:2051-2059 (2019). Read more (PubMed: 30628717) »
- Chen X et al. The Cdc2/Cdk1 inhibitor, purvalanol A, enhances the cytotoxic effects of taxol through Op18/stathmin in non-small cell lung cancer cells in vitro. Int J Mol Med 40:235-242 (2017). Read more (PubMed: 28534969) »