Key features and details
- Rabbit polyclonal to Staufen/STAU1
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Staufen/STAU1 antibody
See all Staufen/STAU1 primary antibodies
DescriptionRabbit polyclonal to Staufen/STAU1
Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Chimpanzee, Rhesus monkey, Orangutan
- This antibody gave a positive signal in the following Human Whole Cell Lysates: SK N BE, SW480
This product was previously labelled as Staufen
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionBinds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
Tissue specificityWidely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.
Sequence similaritiesContains 3 DRBM (double-stranded RNA-binding) domains.
DomainOne of the DRDB could be involved in RER binding.
The C-terminal contains the tubulin binding domain (TBD).
Cellular localizationCytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic.
- Information by UniProt
- Double stranded RNA binding protein Staufen homolog 1 antibody
- Double stranded RNA binding protein Staufen homolog antibody
- Double-stranded RNA-binding protein Staufen homolog 1 antibody
All lanes : Anti-Staufen/STAU1 antibody (ab73478) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Staufen/STAU1 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to Staufen/STAU1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73478.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..
Band: 63kDa: Staufen/STAU1; non specific - 75kDa: We are unsure as to the identity of this extra band.
ICC/IF image of ab73478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
IHC image of Staufen/STAU1 staining in Human Cervical Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
ab73478 has been referenced in 9 publications.
- Koppers M et al. Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. Elife 8:N/A (2019). PubMed: 31746735
- Apicco DJ et al. Reducing the RNA binding protein TIA1 protects against tau-mediated neurodegeneration in vivo. Nat Neurosci 21:72-80 (2018). PubMed: 29273772
- Ravel-Chapuis A et al. Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice. Hum Mol Genet 26:2192-2206 (2017). PubMed: 28369518
- Kobayashi S et al. Local Somatodendritic Translation and Hyperphosphorylation of Tau Protein Triggered by AMPA and NMDA Receptor Stimulation. EBioMedicine 20:120-126 (2017). PubMed: 28566250
- Crawford Parks TE et al. Novel Roles for Staufen1 in Embryonal and Alveolar Rhabdomyosarcoma via c-myc-dependent and -independent events. Sci Rep 7:42342 (2017). PubMed: 28211476
- Bondy-Chorney E et al. Staufen1 Regulates Multiple Alternative Splicing Events either Positively or Negatively in DM1 Indicating Its Role as a Disease Modifier. PLoS Genet 12:e1005827 (2016). WB . PubMed: 26824521
- Pettersson OJ et al. DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1. Nucleic Acids Res 42:7186-200 (2014). PubMed: 24792155
- Ravel-Chapuis A et al. The RNA-binding protein Staufen1 impairs myogenic differentiation via a c-myc-dependent mechanism. Mol Biol Cell 25:3765-78 (2014). WB, IHC-Fr ; Mouse . PubMed: 25208565
- Hubers L et al. HuD interacts with survival motor neuron protein and can rescue spinal muscular atrophy-like neuronal defects. Hum Mol Genet 20:553-79 (2011). WB ; Human . PubMed: 21088113