Overview

  • Product name

    Anti-Staufen/STAU1 antibody [EPR7966]
    See all Staufen/STAU1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7966] to Staufen/STAU1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Staufen/STAU1 aa 550-650. The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, HeLa, Mouse brain, NIH/3T3, Neuro-2a, Rat heart, Jurkat, K562 and HepG2 whole cell lysate (ab7900); ICC/IF: HeLa, Jurkat, and HepG2 cells. FC: SHSY-5Y cells
  • General notes

     This product was previously labelled as Staufen

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 63 kDa).
ICC/IF 1/100.

For unpurified use at 1/250 - 1/500.

Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 For unpurified use at 1/1000 - 1/10000. 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
    • Tissue specificity

      Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.
    • Sequence similarities

      Contains 3 DRBM (double-stranded RNA-binding) domains.
    • Domain

      One of the DRDB could be involved in RER binding.
      The C-terminal contains the tubulin binding domain (TBD).
    • Cellular localization

      Cytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic.
    • Information by UniProt
    • Database links

    • Alternative names

      • Double stranded RNA binding protein Staufen homolog 1 antibody
      • Double stranded RNA binding protein Staufen homolog antibody
      • Double-stranded RNA-binding protein Staufen homolog 1 antibody
      • FLJ25010 antibody
      • MGC124588 antibody
      • PPP1R150 antibody
      • STAU antibody
      • STAU1 antibody
      • STAU1_HUMAN antibody
      • staufen antibody
      • Staufen RNA binding protein (Drosophila) antibody
      • Staufen RNA binding protein homolog 1 antibody
      • Staufen, Drosophila, homolog of, 1 antibody
      • Staufen, RNA binding protein, homolog 1 (Drosophila) antibody
      • staufen-like antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Staufen/STAU1 with Purified ab137100 at 1:100 dilution (5.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Staufen/STAU1 with Purified ab137100 at 1:50 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Anti-Staufen/STAU1 antibody [EPR7966] (ab137100) at 1/1000 dilution (Purified) + Rat heart lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 63 kDa
      Observed band size: 55,63 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-Staufen/STAU1 antibody [EPR7966] (ab137100) at 1/1000 dilution (Purified)

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : Mouse brain lysates
      Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
      Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 63 kDa
      Observed band size: 55,63 kDa why is the actual band size different from the predicted?

    • All lanes : Anti-Staufen/STAU1 antibody [EPR7966] (ab137100) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 63 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      This image was generated using the unpurified version of the product. 

    • Overlay histogram showing SHSY-5Y cells stained with ab137100 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137100, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This image was generated using the unpurified version of the product. 

    • Immunofluorescent analysis of HeLa cells labelling Staufen/STAU1 with ab137100 at 1/250 dilution.

      This image was generated using the unpurified version of the product. 

    • Immunofluorescent analysis of HepG2 cells labelling Staufen/STAU1 with ab137100 at 1/250 dilution.

      This image was generated using the unpurified version of the product. 

    References

    This product has been referenced in:

    • Gershoni-Emek N  et al. Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1. Mol Cell Proteomics 15:506-22 (2016). ICC/IF . Read more (PubMed: 26598648) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (megakaryocytes)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    megakaryocytes
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Jun 07 2013

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Chinese hamster Cell (CHO cell line)
    Permeabilization
    Yes - Triton-X 0.1%
    Specification
    CHO cell line
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Paraformaldehyde

    Dr. Loredana Bury

    Verified customer

    Submitted May 31 2013

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