Recombinant
RabMAb

Recombinant Anti-Staufen/STAU1 antibody [EPR7966] - BSA and Azide free (ab240107)

Overview

  • Product name

    Anti-Staufen/STAU1 antibody [EPR7966] - BSA and Azide free
    See all Staufen/STAU1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7966] to Staufen/STAU1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Staufen/STAU1 aa 550-650. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa, Jurkat, and HepG2 cells. FC: SHSY-5Y cells
  • General notes

    ab240107 is the carrier-free version of ab137100 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240107 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Staufen

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 63 kDa).
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
    • Tissue specificity

      Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.
    • Sequence similarities

      Contains 3 DRBM (double-stranded RNA-binding) domains.
    • Domain

      One of the DRDB could be involved in RER binding.
      The C-terminal contains the tubulin binding domain (TBD).
    • Cellular localization

      Cytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic.
    • Information by UniProt
    • Database links

    • Alternative names

      • Double stranded RNA binding protein Staufen homolog 1 antibody
      • Double stranded RNA binding protein Staufen homolog antibody
      • Double-stranded RNA-binding protein Staufen homolog 1 antibody
      • FLJ25010 antibody
      • MGC124588 antibody
      • PPP1R150 antibody
      • STAU antibody
      • STAU1 antibody
      • STAU1_HUMAN antibody
      • staufen antibody
      • Staufen RNA binding protein (Drosophila) antibody
      • Staufen RNA binding protein homolog 1 antibody
      • Staufen, Drosophila, homolog of, 1 antibody
      • Staufen, RNA binding protein, homolog 1 (Drosophila) antibody
      • staufen-like antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Staufen/STAU1 with Purified ab137100 at 1:100 dilution (5.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol and sodium azide (ab137100).

       

    • Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Staufen/STAU1 with Purified ab137100 at 1:50 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol and sodium azide (ab137100).

       

    • Overlay histogram showing SHSY-5Y cells stained with ab137100 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137100, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137100).

      This image was generated using the unpurified version of the product. 

    • Immunofluorescent analysis of HepG2 cells labelling Staufen/STAU1 with ab137100 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137100).

      This image was generated using the unpurified version of the product. 

    • Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Staufen/STAU1 with ab137100 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol and sodium azide (ab137100).

      This image was generated using the unpurified version of the product.

    References

    ab240107 has not yet been referenced specifically in any publications.

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