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Dear Sir or Madame,
I recently purchased the staurosporine (cat. ab i20056, batch apn0611-3-1-1), dissolved in 100ul DMSO and used for the apptosis induction of Jurkat cells.
Even after using 5uM of the staurosporine no effect on the cells was observed.
Have you experienced any problems with this batch please?
Asked on Apr 05 2012
Thank you for your enquiry and your interest in our products.
We have sold several vials of this product and not received any complaints. Therefore we are not aware of any-quality-related issue regarding ab120056.
It would be much appreciated if I could get some more information which would help me identify the source of the problem.
Q1: Have you carried out some time- and concentration course experiments with ab120056?
Q2: Are the cell still alive after treatment? Have you checked viability?
Q3: Could you please specify what sort of assay you are using to detect apoptotic cells i.e. Casapase (1, 2, 3, 8, 9), Annexin V, DNA ladder, TUNEL?
Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.
Answered on Apr 05 2012