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I used PI and analysed cells on FACS and they were as viable as the cells alone. I hoped that the staurosporine was my positive control, so I did not use anything else.
Asked on Apr 19 2012
Thank you for getting back to me with some further details.
Our Lab is currently undertaking a quality control analysis on this batch of the compound to ensure that it remains of high purity. We expect a result back tomorrow although I expect that there won’t be anything wrong with the compound.
I can offer you a free of charge replacement vial but the vial will be from the same batch since we do not have new batch currently. I would advise you to ensure that a positive control is used to make sure that the assay is working correctly.
Let me know how you wish to proceed. I look forward to hearing from you soon.
Answered on Apr 19 2012