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I used PI and analysed cells on FACS and they were as viable as the cells alone. I hoped that the staurosporine was my positive control, so I did not use anything else.
Asked on Apr 19 2012
Just to confirm that our Lab has finished a quality control (QC) analysis of the compound, which it has passed. We are therefore confident that the compound remains of high purity.
I can certainly offer you a replacement vial and would suggest that you run a positive control to confirm that the assay is running as it should be.
I hope this helps and if I can assist further, please do not hesitate to contact me.
Answered on Apr 19 2012