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StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) (ab178453)

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StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) (ab103743)

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    Overview

    • Product name

      StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible)
      See all StayBlue/AP kits
    • Product overview

      StayBlue/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayBlue/AP produces a vibrant blue color. It is insoluble in alcohol and xylene solutions (both aliphatic and hydrocarbon based) therefore sections can be dehydrated in alcohol, cleared in xylene substitute and permenently mounted.


       


      Working Solution


      Aliquot 3ml of StayBlue/AP substrate buffer in a mixing bottle. Add 20µl (one drop) of concentrated StayBlue/AP chromogen. Replace tip, mix and allow the solution to reach room temperature before using.
      Note: The working chromogen-substrate solution should be prepared fresh and used withing 25 mins of preparation. Any solution not used during this period should be discarded.


       


      Protocol



      • After SA-AP or AP-polymer incubation, wash tissue sections with wash buffer.

      • Wipe slides removing excess buffer. Add enough drops of StayBlue/AP working solution to cover tissue sections.

      • Incubate for 5-25 minutes at room temperature. For optimal results, observe reaction under the microscope for signal development. Once the desired signal to noise ratio is achieved, stop the reaction by rinsing the slides with DI H2O.

      • Note: Increasing incubation temperature to 37°C will increase sensitivity and decrease needed incubation time.

      • Counterstain. Nuclear Fast Red provides good contrast.

      • Wash with DI H2O.

      • Dehydrate sections in alcohol, clear xylene substitute and mount in permanent mounting medium.

      • Note: Use increasing concentrations of ethanol (up to 100%) to dehydrate. Use xylene substitute instead of xylene.

      • Alternatively, slides can be air dried. After rinsing off counterstain in DI H2O, leave the slides on the benchtop for at least 20 minutes to air dry, and then permanently mount.

    • Tested applications

      Suitable for: In situ hybridization, ICCmore details

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 30 ml
      Concentrated StayBlue/AP Chromogen 1 x 1ml
      Empty Mixing Dropping Bottle 1 x 30ml
      StayBlue/AP Substrate Buffer 1 x 30ml
    • Research areas

      • Kits/ Lysates/ Other
      • Tools and Reagents
      • IHC Tools/ Reagents
      • Kits/ Lysates/ Other
      • Kits
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    Associated products

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab178453 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      In situ hybridization
      Use at an assay dependent concentration.
      ICC
      Use at an assay dependent concentration.
      Notes
      In situ hybridization
      Use at an assay dependent concentration.
      ICC
      Use at an assay dependent concentration.

      Images

      • StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) (ab103743)
        StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) (ab103743)

        StayBlue/AP staining CD20 in Human tonsil tissue.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

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      References (0)

      Publishing research using ab178453? Please let us know so that we can cite the reference in this datasheet.

      ab178453 has not yet been referenced specifically in any publications.

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