Product nameStayRed/AP 30ml (Alcohol & Xylene Compatible)
StayRed/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayRed/AP produces a brilliant dark red color. It is also insoluble in organic solvents so sections can be dehydrated in alcohol, cleared in xylene (or a xylene substitute) and permenently mounted.
Substrate/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.
Aliquot 3mL of StayRed/AP Substrate Buffer in a mixing bottle. Add one drop (~20μL) of StayRed/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.
Note: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.
- After SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.
- Wipe slides, removing excess buffer. Add enough drops of StayRed/AP working solution to cover tissue sections.
- Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.
- Counterstain sections in hematoxylin.
- Dehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 30 ml Empty Mixing Dropping Bottle 1 unit StayRed/AP Chromogen 1 x 1ml StayRed/AP Substrate Buffer 1 x 30ml
Formalin fixed paraffin embedded Human tonsil stained with HMW Cytokeratin antibody (ab850) labeled with PermaRed/AP Chromogen (ab103741) produces a brilliant red color.
StayRed staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab103741 has been referenced in 2 publications.
- Aznar MA et al. Immunotherapeutic effects of intratumoral nanoplexed poly I:C. J Immunother Cancer 7:116 (2019). PubMed: 31046839
- Shami A et al. Fibromodulin deficiency reduces low-density lipoprotein accumulation in atherosclerotic plaques in apolipoprotein E-null mice. Arterioscler Thromb Vasc Biol 33:354-61 (2013). PubMed: 23202368