Product nameStayRed/AP 30ml (Alcohol & Xylene Compatible)
StayRed/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayRed/AP produces a brilliant dark red color. It is also insoluble in organic solvents so sections can be dehydrated in alcohol, cleared in xylene (or a xylene substitute) and permenently mounted.
Substrate/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.
Aliquot 3mL of StayRed/AP Substrate Buffer in a mixing bottle. Add one drop (~20μL) of StayRed/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.
Note: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.
- After SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.
- Wipe slides, removing excess buffer. Add enough drops of StayRed/AP working solution to cover tissue sections.
- Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.
- Counterstain sections in hematoxylin.
- Dehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 30 ml Empty Mixing Dropping Bottle 1 unit StayRed/AP Chromogen 1 x 1ml StayRed/AP Substrate Buffer 1 x 30ml
Formalin fixed paraffin embedded Human tonsil stained with HMW Cytokeratin antibody (ab850) labeled with PermaRed/AP Chromogen (ab103741) produces a brilliant red color.
StayRed staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
This product has been referenced in:
- Aznar MA et al. Immunotherapeutic effects of intratumoral nanoplexed poly I:C. J Immunother Cancer 7:116 (2019). Read more (PubMed: 31046839) »
- Shami A et al. Fibromodulin deficiency reduces low-density lipoprotein accumulation in atherosclerotic plaques in apolipoprotein E-null mice. Arterioscler Thromb Vasc Biol 33:354-61 (2013). Read more (PubMed: 23202368) »