Overview

  • Product name
  • Description
    Rabbit polyclonal to STEAP1
  • Host species
    Rabbit
  • Specificity
    This antibody reacts predominantly with STEAP. Ab3679 can be used as a plasma marker for prostate cancer cells
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    WKMKPRRNLEEDDYL

    , corresponding to amino acids 14-28 of Human STEAP.

  • Positive control
    • WB: A431 cell lysate
  • General notes


    Prostate cancer is the most frequently diagnosed cancer and is the second leading cause of cancer-related deaths in American men. Although the early serum detection of prostate-specific antigen (PSA) plays important roles in early diagnosis, it may not distinguish prostate cancer from benign diseases. Recently, a novel protein, which contains 339 amino acids, named STEAP (six transmembrane epithelial antigen of the prostate), was identified in advanced prostate cancer. STEAP is unique among the currently known prostate cancer markers because of its putative secondary structure, from which one may predict that it functions as a potential channel protein. STEAP is strongly expressed in advanced prostate cancer and some extent expression in other cancers, such as colon and ovarian cancer cell lines.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Prostate cancer is the most frequently diagnosed cancer and is the second leading cause of cancer-related deaths in American men. Although the early serum detection of prostate-specific antigen (PSA) plays important roles in early diagnosis, it may not distinguish prostate cancer from benign diseases. Recently, a novel protein, which contains 339 amino acids, named STEAP (six transmembrane epithelial antigen of the prostate), was identified in advanced prostate cancer. STEAP is unique among the currently known prostate cancer markers because of its putative secondary structure, from which one may predict that it functions as a potential channel protein. STEAP is strongly expressed in advanced prostate cancer and some extent expression in other cancers, such as colon and ovarian cancer cell lines.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.
  • Tissue specificity
    Ubiquitously expressed. Highly expressed in prostate tumors.
  • Sequence similarities
    Belongs to the STEAP family.
    Contains 1 ferric oxidoreductase domain.
  • Cellular localization
    Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410007B19Rik antibody
    • EC 1.16.1.- antibody
    • Metalloreductase STEAP1 antibody
    • MGC19484 antibody
    • PRSS 24 antibody
    • PRSS24 antibody
    • Six transmembrane epithelial antigen of prostate antibody
    • Six transmembrane epithelial antigen of the prostate 1 antibody
    • Six transmembrane epithelial antigen of the prostate antibody
    • Six-transmembrane epithelial antigen of prostate 1 antibody
    • STEA1_HUMAN antibody
    • STEAP 1 antibody
    • Steap antibody
    • STEAP1 antibody
    see all

Images

  •  A431 cell lysate probed with anti-STEAP (ab3679) at 1 microgram/ml

    A431 cell lysate probed with anti-STEAP (ab3679) at 1 microgram/ml

References

ab3679 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question

Sorry for the delay, however I just heard back from the customer after several contact attempts. I have attached their answers to your questions. In answer to your questions: 1) We see many non specific bands (please see picture attached for a sample) 2) Antibody is being tested on EL-4 (mouse T cells) that have been transfected with pcDNA3 encoded with human STEAP. WB was done on whole cell extract. 3) Sample were lysed with SDS, protease inhibitors, b-mercapto, EDTA, etc. Samples were heated for 10 min at 100'C. 4) Ab was raised against Human STEAP. Dilutions used were 1/500, 1/1000, 1/2000, 1/6000. Incubation time of 1hour followed by three thorough washing steps. 5) Secondary antibody is an anti-rabbit. Incubated for 45 min followed by three thorough washing steps. Secondary antibody is commonly used by numerous members of our lab with no problems. 6) ECL kit. 7) Secondary antibody has been run alone (with no primary antibody) and shows no background whatsoever. Blocking and washing steps are sufficient and stringent. We block o/n with agitation at 4 degrees. Washing is has been done 3 times for 10 min each, or 6 times for 5 min each. Bands cannot be degraded products as they are at a higher molecular weight then the full-length expected Band size. 8) Westerns were done many times with the same results. Altered steps were washing times, antibody concentration, altered incubation times. 9) Negative controls showed the same result to a lesser intensity.no positive controls were used. Just to recap, this is the customer that found the same result with his transfected and non-transfected cells (control) and saw no difference in staining. He does not get the 40kDa band that he expects to see (I will ask to see if the customer can show the MWts of the control bands), but a lot of other bands instead. Customer is very unhappy and wants a credit. I look forward to your response,

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Answer

Thank you for your enquiry and your patience. We are very sorry that the customer is having problem with this product. We have read through the forwarded protocol and it seems to be fine. After careful consideration, we offer a credit note. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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