Product nameSTEAP1 overexpression 293T lysate (whole cell)
DescriptionSTEAP1 overexpression lysate
ab94255 is a 293T cell transfected lysate in which Human STEAP1 has been transiently over-expressed using a pCMV-STEAP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Tissue specificity: Ubiquitously expressed. Highly expressed in prostate tumors. Similarity: Belongs to the STEAP family. Contains 1 ferric oxidoreductase domain.
Our Abpromise guarantee covers the use of ab94255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94255 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-STEAP1 antibody (ab57147) at 1/500 dilution
Lane 1 :
STEAP1 overexpression 293T lysate (whole cell) (ab94255)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94255 has not yet been referenced specifically in any publications.