Anti-STEAP3 antibody (ab104654)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-STEAP3 antibody
See all STEAP3 primary antibodies -
Description
Rabbit polyclonal to STEAP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse STEAP3 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab130906) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Liver; Mouse Brain; Mouse Heart. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: JEG3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab104654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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Function
Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP. -
Tissue specificity
Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma. -
Involvement in disease
Anemia, hypochromic microcytic, with iron overload 2 -
Sequence similarities
Belongs to the STEAP family.
Contains 1 ferric oxidoreductase domain. -
Post-translational
modificationsProteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.
Glycosylated. -
Cellular localization
Endosome membrane. Localizes to vesicular-like structures at the plasma membrane and around the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 68428 Mouse
- SwissProt: Q8CI59 Mouse
- Unigene: 181033 Mouse
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Alternative names
- 1010001D01Rik antibody
- Dudlin 2 antibody
- Dudlin2 antibody
see all
Images
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Western blot - Anti-STEAP3 antibody (ab104654)All lanes : Anti-STEAP3 antibody (ab104654) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 105 kDa, 130 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-STEAP3 antibody (ab104654)ICC/IF image of ab104654 stained JEG3 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104654 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
References
ab104654 has not yet been referenced specifically in any publications.
