Product nameAnti-STEAP3 antibody [EPR9812]
See all STEAP3 primary antibodies
DescriptionRabbit monoclonal [EPR9812] to STEAP3
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human STEAP3 aa 50-150. The exact sequence is proprietary.
- HeLa, HepG2, PC12 and C6 cell lysates; HepG2 cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab151566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 54 kDa.|
|ICC/IF||1/250 - 1/500.|
FunctionEndosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.
Tissue specificityExpressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.
Involvement in diseaseAnemia, hypochromic microcytic, with iron overload 2
Sequence similaritiesBelongs to the STEAP family.
Contains 1 ferric oxidoreductase domain.
modificationsProteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.
Cellular localizationEndosome membrane. Localizes to vesicular-like structures at the plasma membrane and around the nucleus.
- Information by UniProt
- 1010001D01Rik antibody
- Dudlin 2 antibody
- Dudlin2 antibody
All lanes : Anti-STEAP3 antibody [EPR9812] (ab151566) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
Immunofluorescent analysis of HepG2 cells labeling STEAP3 with ab151566 at 1/250 dilution.
ab151566 has not yet been referenced specifically in any publications.