Key features and details
- Chicken polyclonal to STELLAR
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgY
Product nameAnti-STELLAR antibody
See all STELLAR primary antibodies
DescriptionChicken polyclonal to STELLAR
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Human STELLAR aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
- In western blot, ab136278 gave a positive signal in the following whole cell lysates: mouse embryonic germ cells; E14Tg2a. This antibody gave a positive result when used in the following methanol fixed cell lines: Mouse embryonic stem cells.
This product was previously labelled as Stella
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab136278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMay play a role in maintaining cell pluripotentiality.
Tissue specificityLow expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
Developmental stageExpressed in fetal ovary.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
FormStella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage.
- Compaction associated protein 1 antibody
- Developmental pluripotency associated 3 antibody
- Developmental pluripotency associated protein 3 antibody
All lanes : Anti-STELLAR antibody (ab136278) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate (ab59325)
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab136278 stained Mouse Embryonic Stem cells cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab136278 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab136278 has not yet been referenced specifically in any publications.