Overview

  • Product name

  • Description

    Chicken polyclonal to STELLAR
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Human STELLAR aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.

  • Positive control

    • In western blot, ab136278 gave a positive signal in the following whole cell lysates: mouse embryonic germ cells; E14Tg2a. This antibody gave a positive result when used in the following methanol fixed cell lines: Mouse embryonic stem cells.
  • General notes

     This product was previously labelled as Stella

     

Properties

Applications

Our Abpromise guarantee covers the use of ab136278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    May play a role in maintaining cell pluripotentiality.
  • Tissue specificity

    Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
  • Developmental stage

    Expressed in fetal ovary.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Form

    Stella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage.
  • Alternative names

    • Compaction associated protein 1 antibody
    • Developmental pluripotency associated 3 antibody
    • Developmental pluripotency associated protein 3 antibody
    • Developmental pluripotency-associated protein 3 antibody
    • Dppa3 antibody
    • DPPA3_HUMAN antibody
    • PGC7 antibody
    • Stella related protein antibody
    • Stella-related protein antibody
    see all

Images

  • All lanes : Anti-STELLAR antibody (ab136278) at 1 µg/ml

    Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 2 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate (ab59325)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • ab136278 stained Mouse Embryonic Stem cells cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab136278 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab136278 has not yet been referenced specifically in any publications.

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