Overview

  • Product name

  • Description

    Rabbit polyclonal to STELLAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human STELLAR (C terminal).
    Database link: NP_954980

  • Positive control

    • 293 cell lysate
  • General notes

     This product was previously labelled as Stella

     

Properties

Applications

Our Abpromise guarantee covers the use of ab74531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Target

  • Function

    May play a role in maintaining cell pluripotentiality.
  • Tissue specificity

    Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
  • Developmental stage

    Expressed in fetal ovary.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Form

    Stella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage.
  • Alternative names

    • Compaction associated protein 1 antibody
    • Developmental pluripotency associated 3 antibody
    • Developmental pluripotency associated protein 3 antibody
    • Developmental pluripotency-associated protein 3 antibody
    • Dppa3 antibody
    • DPPA3_HUMAN antibody
    • PGC7 antibody
    • Stella related protein antibody
    • Stella-related protein antibody
    see all

Images

  • Lane 1 : Anti-STELLAR antibody (ab74531) at 1 µg/ml
    Lane 2 : Anti-STELLAR antibody (ab74531) at 2 µg/ml

    All lanes : 293 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

References

This product has been referenced in:

  • Hu X  et al. Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro. Int J Mol Med 35:1219-29 (2015). Read more (PubMed: 25760093) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Gonad)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization
Yes - 0.05% triton X in PBS
Specification
Mouse Gonad
Fixative
Paraformaldehyde

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Verified customer

Submitted Nov 24 2017

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