Anti-Steroid sulfatase antibody (ab233233)
Key features and details
- Rabbit polyclonal to Steroid sulfatase
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Steroid sulfatase antibody -
Description
Rabbit polyclonal to Steroid sulfatase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Steroid sulfatase aa 200 to the C-terminus. Expressed in E. coli. N-terminal tags. 2nd Lumenal domain.
Database link: P08842 -
Positive control
- IHC-P: Human liver tissue. WB: Recombinant human Steroid sulfatase protein. Mouse testis and placenta lysate; Human serum.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa. |
Target
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Function
Conversion of sulfated steroid precursors to estrogens during pregnancy. -
Involvement in disease
Defects in STS are the cause of ichthyosis X-linked (IXL) [MIM:308100]. Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks. -
Sequence similarities
Belongs to the sulfatase family. -
Post-translational
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 412 Human
- Entrez Gene: 20905 Mouse
- Omim: 308100 Human
- SwissProt: P08842 Human
- SwissProt: P50427 Mouse
- Unigene: 522578 Human
- Unigene: 700558 Human
- Unigene: 700559 Human
see all -
Alternative names
- ARSC antibody
- ARSC1 antibody
- Arylsulfatase C antibody
see all
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for Steroid sulfatase with ab233233 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Steroid sulfatase antibody (ab233233) at 1 µg/ml + Mouse testis tissue lysate
Predicted band size: 65 kDa -
Anti-Steroid sulfatase antibody (ab233233) at 1 µg/ml + Human serum
Predicted band size: 65 kDa -
Anti-Steroid sulfatase antibody (ab233233) at 1 µg/ml + Mouse placenta tissue lysate
Predicted band size: 65 kDa -
Anti-Steroid sulfatase antibody (ab233233) at 1 µg/ml + Recombinant human Steroid sulfatase protein
Predicted band size: 65 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233233 has not yet been referenced specifically in any publications.