• Product name

    Anti-Steroid sulfatase antibody
    See all Steroid sulfatase primary antibodies
  • Description

    Rabbit polyclonal to Steroid sulfatase
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Cow
  • Immunogen

    A 14-22 amino acid region within synthetic peptide: ISKDPRERNP LTPASEPRFY EILKVMQEAA DRHTQTLPEV PDQFSWNNFL, corresponding to C terminal amino acids 505-554 of Human Steroid sulfatase

  • Positive control

    • Placental cell lysate


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab62219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function

    Conversion of sulfated steroid precursors to estrogens during pregnancy.
  • Involvement in disease

    Defects in STS are the cause of ichthyosis X-linked (IXL) [MIM:308100]. Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks.
  • Sequence similarities

    Belongs to the sulfatase family.
  • Post-translational

    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARSC antibody
    • ARSC1 antibody
    • Arylsulfatase C antibody
    • Arylsulfatase C isozyme S antibody
    • ASC antibody
    • EC antibody
    • ES antibody
    • Estrone sulfatase antibody
    • SSDD antibody
    • Steroid sulfatase (microsomal) antibody
    • Steroid sulfatase (microsomal) arylsulfatase C isozyme S antibody
    • Steroid sulfatase antibody
    • Steryl sulfatase antibody
    • Steryl sulfatase precursor antibody
    • Steryl sulfate sulfohydrolase antibody
    • Steryl-sulfatase antibody
    • Steryl-sulfate sulfohydrolase antibody
    • STS antibody
    • STS_HUMAN antibody
    see all


  • Anti-Steroid sulfatase antibody (ab62219) at 5 µg/ml + Placental cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • IHC image of Stroid sulfatase staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab62219, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


This product has been referenced in:

  • Lee WM  et al. The Role of Steroid Sulfatase as a Prognostic Factor in Patients with Endometrial Cancer. Yonsei Med J 57:754-60 (2016). IHC-P . Read more (PubMed: 26996578) »
  • Jiang M  et al. Hepatic overexpression of steroid sulfatase ameliorates mouse models of obesity and type 2 diabetes through sex-specific mechanisms. J Biol Chem 289:8086-97 (2014). WB ; Mouse . Read more (PubMed: 24497646) »
See all 3 Publications for this product

Customer reviews and Q&As


Thank you for contacting us. The black dots on a blot usually indicate that the antibody is binding to the blocking reagent and it needs to be filtered. The 5% milk should be in TBST. I know you have said that you have used your protocol sucessfully with other antibodies, but maybe filtering again would improve your results. I would recommend diluting the primary in 5% milk in TBST rather than just PBST or TBST. Please let me know if this problem is not resolved by these protocol tips.

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