Key features and details
- Rabbit polyclonal to Steroidogenic Factor 1/SF-1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Steroidogenic Factor 1/SF-1 antibody
See all Steroidogenic Factor 1/SF-1 primary antibodies
DescriptionRabbit polyclonal to Steroidogenic Factor 1/SF-1
SpecificityBLAST analysis of the peptide immunogen showed no homology with other human proteins, except TRIM23 (47%).
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla
Synthetic peptide corresponding to Human Steroidogenic Factor 1/SF-1 (internal sequence). (17 amino acid peptide).
Database link: Q13285
- Human ovary tissue.
Previously labelled as Steroidogenic Factor 1.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.099% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 18 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionTranscriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.
Involvement in diseaseDefects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
modificationsAcetylation stimulates the transcriptional activity.
- Information by UniProt
- AD4BP antibody
- Adrenal 4 binding protein antibody
- Adrenal 4-binding protein antibody
ab219500 has not yet been referenced specifically in any publications.