Key features and details
- Rabbit polyclonal to Steroidogenic Factor 1/SF-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Steroidogenic Factor 1/SF-1 antibody
See all Steroidogenic Factor 1/SF-1 primary antibodies
DescriptionRabbit polyclonal to Steroidogenic Factor 1/SF-1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Pig
- Recombinant Human Steroidogenic Factor 1/SF-1 protein (ab114599) can be used as a positive control in WB. This antibody gave a positive signal in the following human tissue lysates: Testis; Ovary; Thymus.
Previously labelled as Steroidogenic Factor 1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionTranscriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.
Involvement in diseaseDefects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
modificationsAcetylation stimulates the transcriptional activity.
- Information by UniProt
- AD4BP antibody
- Adrenal 4 binding protein antibody
- Adrenal 4-binding protein antibody
All lanes : Anti-Steroidogenic Factor 1/SF-1 antibody (ab65815) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein
Lane 2 : Human ovary tissue lysate - total protein
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
Exposure time: 16 minutes
Steroidogenic Factor 1/SF-1 contains a potential phosphorylation site, as well as a number of acetylation sites(SwissProt), which may explain its migration at a higher molecular weight than predicted. The 55 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to this target.
COS-1 whole cell lysate added at 20µg.
Primary antibody used at a 1/1000 dilution.Secondary antibody used was a goat anti-rabbit IgG/ HRP at a 1/2500 dilution.
IHC-P image of Steroidogenic Factor 1/SF-1 (ab65815) on Human testis sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 mins at 22°C.
All lanes : Anti-Steroidogenic Factor 1/SF-1 antibody (ab65815) at 1/900 dilution
Lane 1 : Canine adrenocortical tumour whole cell lysate with ECL Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Lane 2 : canine normal adrenal whole cell lysate with ECL Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Lane 3 : H295R whole cell lysate with ECL Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
All lanes : Goat anti-Rabbit IgG, at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Formalin-fixed, paraffin-embedded dog adrenal cortex tissue stained for Steroidogenic Factor 1/SF-1 using ab65815 at 1/300 dilution in immunohistochemical analysis.
ab65815 has been referenced in 4 publications.
- Wu PL et al. Transcription factor 21 regulates expression of ERß and SF-1 via upstream stimulatory factor-2 in endometriotic tissues. Biochim Biophys Acta Gene Regul Mech 1861:706-717 (2018). PubMed: 30018006
- Lu Y et al. Epigenetic modifications promote the expression of the orphan nuclear receptor NR0B1 in human lung adenocarcinoma cells. Oncotarget 7:43162-43176 (2016). PubMed: 27281610
- El Hajj Chehadeh S et al. Early methyl donor deficiency alters cAMP signaling pathway and neurosteroidogenesis in the cerebellum of female rat pups. Am J Physiol Endocrinol Metab 307:E1009-19 (2014). PubMed: 25294213
- Bertolin K et al. The orphan nuclear receptor Nr5a2 is essential for luteinization in the female mouse ovary. Endocrinology 155:1931-43 (2014). PubMed: 24552399