RecombinantRabMAb

Recombinant Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)

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Western blot - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)
  • Western blot - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)
  • Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11695(B)] to Steroidogenic Factor 1/SF-1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free
    See all Steroidogenic Factor 1/SF-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11695(B)] to Steroidogenic Factor 1/SF-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Steroidogenic Factor 1/SF-1. The exact sequence is proprietary.
    Database link: Q13285

  • Positive control

    • Rat and mouse brain, Jurkat and HepG2 whole cell lysate (ab7900).

  • General notes

    Ab249471 is the carrier-free version of ab168380. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249471 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.

    • Involvement in disease

      Defects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
      Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
      Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR5 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • Post-translational
      modifications

      Acetylation stimulates the transcriptional activity.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AD4BP antibody
      • Adrenal 4 binding protein antibody
      • Adrenal 4-binding protein antibody
      • ELP antibody
      • FTZ 1 antibody
      • FTZ1 antibody
      • FTZF 1 antibody
      • FTZF1 antibody
      • Fushi tarazu factor (Drosophila) homolog 1 antibody
      • Fushi tarazu factor homolog 1 antibody
      • hSF 1 antibody
      • NR5A1 antibody
      • Nuclear receptor AdBP4 antibody
      • Nuclear receptor subfamily 5 group A member 1 antibody
      • POF7 antibody
      • SF 1 antibody
      • SF-1 antibody
      • SF1 antibody
      • SPGF8 antibody
      • SRXY3 antibody
      • Steroid hormone receptor Ad4BP antibody
      • Steroidogenic factor 1 antibody
      • Steroidogenic factor 1 nuclear receptor antibody
      • STF 1 antibody
      • STF-1 antibody
      • STF1 antibody
      • STF1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)
      Western blot - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)
      All lanes : Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] (ab168380) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 52 kDa

    • Western blot - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)
      Western blot - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)
      All lanes : Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] (ab168380) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa



      This data was developed using ab168380, the same antibody clone in a different buffer formulation.

       

    • Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)
      Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] - BSA and Azide free (ab249471)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab249471? Please let us know so that we can cite the reference in this datasheet.

    ab249471 has not yet been referenced specifically in any publications.

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