Recombinant
RabMAb

Recombinant Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)

Rabbit recombinant monoclonal Steroidogenic Factor 1 / SF-1 antibody [EPR19744]. Validated in IHC and tested in Human.

Overview

  • Product name

    Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744]
    See all Steroidogenic Factor 1/SF-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19744] to Steroidogenic Factor 1/SF-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Steroidogenic Factor 1/SF-1 aa 200-300. The exact sequence is proprietary.
    Database link: Q13285

  • Positive control

    • IHC-P: Human ovary, testis and adrenal cancer tissues.
  • General notes

    Previously labelled as Steroidogenic Factor 1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab217317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.
  • Involvement in disease

    Defects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
    Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
    Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    modifications

    Acetylation stimulates the transcriptional activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AD4BP antibody
    • Adrenal 4 binding protein antibody
    • Adrenal 4-binding protein antibody
    • ELP antibody
    • FTZ 1 antibody
    • FTZ1 antibody
    • FTZF 1 antibody
    • FTZF1 antibody
    • Fushi tarazu factor (Drosophila) homolog 1 antibody
    • Fushi tarazu factor homolog 1 antibody
    • hSF 1 antibody
    • NR5A1 antibody
    • Nuclear receptor AdBP4 antibody
    • Nuclear receptor subfamily 5 group A member 1 antibody
    • POF7 antibody
    • SF 1 antibody
    • SF-1 antibody
    • SF1 antibody
    • SPGF8 antibody
    • SRXY3 antibody
    • Steroid hormone receptor Ad4BP antibody
    • Steroidogenic factor 1 antibody
    • Steroidogenic factor 1 nuclear receptor antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    • STF1_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human ovary is observed [PMID: 20660055] [PMID: 10370224]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human testis is observed [PMID: 10370224]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human adrenal cancer tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human adrenal cancer is observed [PMID: 20660055]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab217317 has not yet been referenced specifically in any publications.

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