Recombinant Anti-Sterol carrier protein 2 antibody [EPR9022] (ab140126)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9022] to Sterol carrier protein 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Sterol carrier protein 2 antibody [EPR9022]
See all Sterol carrier protein 2 primary antibodies -
Description
Rabbit monoclonal [EPR9022] to Sterol carrier protein 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human Sterol carrier protein 2 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WI38 and HepG2 whole cell lysate (ab7900); Human placenta and Mouse liver tissues; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9022 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. May play a role in regulating steroidogenesis. -
Tissue specificity
Liver, fibroblasts, and placenta. -
Involvement in disease
Defects in SCP2 are a cause of leukoencephalopathy with dystonia and motor neuropathy (LDMN) [MIM:613724]; also known as sterol carrier protein 2 deficiency. LDMN is a syndrome characterized by leukoencephalopathy, dystonic head tremor, spasmodic torticollis and reduced tendon reflexes in lower extremities. Additional features include hyposmia, pathologic saccadic eye movements, a slight hypoacusis, accumulation of branched-chain pristanic acid in plasma, and the presence of abnormal bile alcohol glucuronides in urine. -
Sequence similarities
In the N-terminal section; belongs to the thiolase family.
Contains 1 SCP2 domain. -
Cellular localization
Mitochondrion; Cytoplasm. Mitochondrion. Cytoplasmic in the liver and also associated with mitochondria especially in steroidogenic tissues and Peroxisome. Interaction with PEX5 is essential for peroxisomal import. - Information by UniProt
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Database links
- Entrez Gene: 6342 Human
- Entrez Gene: 20280 Mouse
- Entrez Gene: 25541 Rat
- Omim: 184755 Human
- SwissProt: P22307 Human
- SwissProt: P32020 Mouse
- SwissProt: P11915 Rat
- Unigene: 476365 Human
see all -
Alternative names
- DKFZp686C12188 antibody
- DKFZp686D11188 antibody
- NLTP antibody
see all
Images
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Immunohistochemical analysis of Paraffin-embedded Human placenta tissue labelling Sterol carrier protein 2 with ab140126 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Sterol carrier protein 2 antibody [EPR9022] (ab140126) at 1/1000 dilution
Lane 1 : WI38 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa -
Immunohistochemical analysis of Paraffin-embedded Mouse liver tissue labelling Sterol carrier protein 2 with ab140126 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent analysis of HepG2 cells labelling Sterol carrier protein 2 with ab140126 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab140126 has been referenced in 6 publications.
- Gao K et al. Qishen granules exerts cardioprotective effects on rats with heart failure via regulating fatty acid and glucose metabolism. Chin Med 15:21 (2020). PubMed: 32158496
- Ding X et al. SCP2-mediated cholesterol membrane trafficking promotes the growth of pituitary adenomas via Hedgehog signaling activation. J Exp Clin Cancer Res 38:404 (2019). PubMed: 31519191
- Dai W et al. Understanding the regulatory mechanisms of milk production using integrative transcriptomic and proteomic analyses: improving inefficient utilization of crop by-products as forage in dairy industry. BMC Genomics 19:403 (2018). PubMed: 29843597
- Zhang Q et al. The Effect of Chinese Medicine on Lipid and Glucose Metabolism in Acute Myocardial Infarction Through PPAR? Pathway. Front Pharmacol 9:1209 (2018). PubMed: 30405421
- Fan J et al. ACBD2/ECI2-Mediated Peroxisome-Mitochondria Interactions in Leydig Cell Steroid Biosynthesis. Mol Endocrinol 30:763-82 (2016). PubMed: 27167610
- Li NC et al. Sterol Carrier Protein-2, a Nonspecific Lipid-Transfer Protein, in Intracellular Cholesterol Trafficking in Testicular Leydig Cells. PLoS One 11:e0149728 (2016). PubMed: 26901662