Overview

  • Product name

  • Description

    Rabbit polyclonal to STIL/SIL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse STIL/SIL aa 1050-1150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab138803)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Thymus; Rat Thymus; Mouse Bone Marrow; Mouse Spleen; Rat Spleen.
  • General notes

     This product was previously labelled as STIL

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 138 kDa).

Target

  • Function

    Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Play a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.
  • Tissue specificity

    Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
  • Involvement in disease

    Note=A chromosomal aberration involving STIL may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). A deletion at 1p32 between STIL and TAL1 genes leads to STIL/TAL1 fusion mRNA with STIL exon 1 slicing to TAL1 exon 3. As both STIL exon 1 and TAL1 exon 3 are 5'-untranslated exons, STIL/TAL1 fusion mRNA predicts a full length TAL1 protein under the control of the STIL promoter, leading to inappropriate TAL1 expression. In childhood T-cell malignancies (T-ALL), a type of defect such as STIL/TAL1 fusion is associated with a good prognosis. In cultured lymphocytes from healthy adults, STIL/TAL1 fusion mRNA may be detected after 7 days of culture.
    Defects in STIL are the cause of microcephaly primary type 7 (MCPH7) [MIM:612703]. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
  • Post-translational
    modifications

    Phosphorylated following the activation of the mitotic checkpoint.
  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686O09161 antibody
    • MCPH7 antibody
    • OTTHUMP00000009566 antibody
    • SCL interrupting locus protein antibody
    • SCL-interrupting locus protein antibody
    • SCL/TAL1 interrupting locus antibody
    • SIL antibody
    • STIL antibody
    • STIL_HUMAN antibody
    • TAL 1 interrupting locus protein antibody
    • TAL-1-interrupting locus protein antibody
    see all

Images

  • All lanes : Anti-STIL/SIL antibody (ab124322) at 1 µg/ml

    Lane 1 : Thymus (Mouse) Tissue Lysate
    Lane 2 : Thymus (Rat) Tissue Lysate
    Lane 3 : Mouse Bone Marrow Tissue Lysate
    Lane 4 : Spleen (Mouse) Tissue Lysate
    Lane 5 : Spleen (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 138 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 14 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

References

ab124322 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab124322.
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