Product nameAnti-STIL/SIL antibody
See all STIL/SIL primary antibodies
DescriptionRabbit polyclonal to STIL/SIL
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide corresponding to Human STIL/SIL aa 1237-1287.
Database link: NP_003026.2
This product was previously labelled as STIL
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab89314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 143 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionImmediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Play a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.
Tissue specificityExpressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
Involvement in diseaseNote=A chromosomal aberration involving STIL may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). A deletion at 1p32 between STIL and TAL1 genes leads to STIL/TAL1 fusion mRNA with STIL exon 1 slicing to TAL1 exon 3. As both STIL exon 1 and TAL1 exon 3 are 5'-untranslated exons, STIL/TAL1 fusion mRNA predicts a full length TAL1 protein under the control of the STIL promoter, leading to inappropriate TAL1 expression. In childhood T-cell malignancies (T-ALL), a type of defect such as STIL/TAL1 fusion is associated with a good prognosis. In cultured lymphocytes from healthy adults, STIL/TAL1 fusion mRNA may be detected after 7 days of culture.
Defects in STIL are the cause of microcephaly primary type 7 (MCPH7) [MIM:612703]. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
modificationsPhosphorylated following the activation of the mitotic checkpoint.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- DKFZp686O09161 antibody
- MCPH7 antibody
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All lanes : Anti-STIL/SIL antibody (ab89314) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 143 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
ICC/IF image of ab89314 stained T24/83 cells. The cells were 4% paraformaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89314, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
Detection of STIL/SIL in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab89314 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-STIL/SIL antibody (ab89314) at 0.1 µg/ml
Lane 1 : TCMK-1 cell lysate
Lane 2 : 4T1 cell lysate
Lane 3 : CT26.WT cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 143 kDa
Exposure time: 3 minutes
This product has been referenced in:
- Yamamoto S & Kitagawa D Self-organization of Plk4 regulates symmetry breaking in centriole duplication. Nat Commun 10:1810 (2019). Read more (PubMed: 31000710) »
- Nanjundappa R et al. Regulation of cilia abundance in multiciliated cells. Elife 8:N/A (2019). Read more (PubMed: 31025935) »