Overview

  • Product name

  • Description

    Rabbit polyclonal to STIL/SIL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human STIL/SIL aa 1237-1287.
    Database link: NP_003026.2

  • Positive control

    • WB: HeLa whole cell lysate (ab150035). IP: HeLa whole cell lysate (ab150035). ICC/IF: T24/83 cell line.
  • General notes

     This product was previously labelled as STIL

     

Properties

Applications

Our Abpromise guarantee covers the use of ab89314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Play a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.
  • Tissue specificity

    Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
  • Involvement in disease

    Note=A chromosomal aberration involving STIL may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). A deletion at 1p32 between STIL and TAL1 genes leads to STIL/TAL1 fusion mRNA with STIL exon 1 slicing to TAL1 exon 3. As both STIL exon 1 and TAL1 exon 3 are 5'-untranslated exons, STIL/TAL1 fusion mRNA predicts a full length TAL1 protein under the control of the STIL promoter, leading to inappropriate TAL1 expression. In childhood T-cell malignancies (T-ALL), a type of defect such as STIL/TAL1 fusion is associated with a good prognosis. In cultured lymphocytes from healthy adults, STIL/TAL1 fusion mRNA may be detected after 7 days of culture.
    Defects in STIL are the cause of microcephaly primary type 7 (MCPH7) [MIM:612703]. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
  • Post-translational
    modifications

    Phosphorylated following the activation of the mitotic checkpoint.
  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686O09161 antibody
    • MCPH7 antibody
    • OTTHUMP00000009566 antibody
    • SCL interrupting locus protein antibody
    • SCL-interrupting locus protein antibody
    • SCL/TAL1 interrupting locus antibody
    • SIL antibody
    • STIL antibody
    • STIL_HUMAN antibody
    • TAL 1 interrupting locus protein antibody
    • TAL-1-interrupting locus protein antibody
    see all

Images

  • All lanes : Anti-STIL/SIL antibody (ab89314) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 143 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • ICC/IF image of ab89314 stained T24/83 cells. The cells were 4% paraformaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89314, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

  • Detection of STIL/SIL in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab89314 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 30 seconds.

  • All lanes : Anti-STIL/SIL antibody (ab89314) at 0.1 µg/ml

    Lane 1 : TCMK-1 cell lysate
    Lane 2 : 4T1 cell lysate
    Lane 3 : CT26.WT cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 143 kDa


    Exposure time: 3 minutes

References

This product has been referenced in:

See all 18 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam. I am sorry that you are having issues with ab89314 in western blot. Having looked over the data and protocol information you kindly sent, I have a few suggestions that may help: 1 - Although the predicted mol weight is 143kDa, the western blot data on our website shows that when the antibody is used against HeLa cell extract, it recognizes a band at 170kDa. From the image you provided there does appear to be a (faint) band at about 170kDa, which may be correct. 2 - There are a lot of non-specific bands in your image; one reason for this could be breakdown of your protein, as I noticed you were not used protease inhibitors in your lysis buffer. I would advise adding protease inhibitors to your buffer. 3 - An alternative explanation for the multiply bands, could be that the antibody is binding non-specifically and therefore I would recommend switching to a 5% BSA block and antibody incubation buffer. 4 - I would also recommend loading more of your HeLa cell sample into your gel. Currently you are loading 3ul od samples, I suggest increasing this to 10ul, which will increase the signal from the 170kDa band. I hope that these protocol tips prove to be helpful. If not under our Abpromise, this antibody is guaranteed to work in western blot on human tissue. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund. If there is anything else I can help you with, plea

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