Overview

  • Product name

  • Description

    Rabbit polyclonal to STIM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human STIM2 aa 471-656.
    Database link: Q9P246

  • Positive control

    • WB: HepG2 and Jurkat cell lysates; Mouse heart tissue lysate. IHC-P: Human brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 69,84 kDa (predicted molecular weight: 69,84,94 kDa).
IHC-P 1/20 - 1/200.

Target

  • Function

    Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
  • Tissue specificity

    Expressed in all tissues and tumor cell lines examined.
  • Sequence similarities

    Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications

    Glycosylated.
    Phosphorylated predominantly on Ser residues.
  • Cellular localization

    Endoplasmic reticulum membrane. Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ39527 antibody
    • KIAA1482 antibody
    • STIM 2 antibody
    • STIM2 antibody
    • STIM2_HUMAN antibody
    • Stromal interaction molecule 2 antibody
    see all

Images

  • Paraffin-embedded human brain tissue stained for STIM2 using ab223033 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-STIM2 antibody (ab223033) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : Mouse heart tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 69,84,94 kDa
    Observed band size: 69,84 kDa
    why is the actual band size different from the predicted?

References

ab223033 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223033.
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