Anti-STIM2 antibody (ab223033)
Key features and details
- Rabbit polyclonal to STIM2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-STIM2 antibody
See all STIM2 primary antibodies -
Description
Rabbit polyclonal to STIM2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human STIM2 aa 471-656.
Database link: Q9P246 -
Positive control
- WB: HepG2 and Jurkat cell lysates; Mouse heart tissue lysate. IHC-P: Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 69,84 kDa (predicted molecular weight: 69,84,94 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 69,84 kDa (predicted molecular weight: 69,84,94 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Function
Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx. -
Tissue specificity
Expressed in all tissues and tumor cell lines examined. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsGlycosylated.
Phosphorylated predominantly on Ser residues. -
Cellular localization
Endoplasmic reticulum membrane. Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration. - Information by UniProt
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Database links
- Entrez Gene: 57620 Human
- Entrez Gene: 116873 Mouse
- Omim: 610841 Human
- SwissProt: Q9P246 Human
- SwissProt: P83093 Mouse
- Unigene: 726179 Human
- Unigene: 265985 Mouse
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Alternative names
- FLJ39527 antibody
- KIAA1482 antibody
- STIM 2 antibody
see all
Images
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Paraffin-embedded human brain tissue stained for STIM2 using ab223033 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-STIM2 antibody (ab223033) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : Mouse heart tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 69,84,94 kDa
Observed band size: 69,84 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223033 has not yet been referenced specifically in any publications.