Product nameAnti-STIM2 antibody
See all STIM2 primary antibodies
DescriptionRabbit polyclonal to STIM2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human STIM2 aa 471-656.
Database link: Q9P246
- WB: HepG2 and Jurkat cell lysates; Mouse heart tissue lysate. IHC-P: Human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab223033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 69,84 kDa (predicted molecular weight: 69,84,94 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionFunctions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
Tissue specificityExpressed in all tissues and tumor cell lines examined.
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain.
Phosphorylated predominantly on Ser residues.
Cellular localizationEndoplasmic reticulum membrane. Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration.
- Information by UniProt
- FLJ39527 antibody
- KIAA1482 antibody
- STIM 2 antibody
Paraffin-embedded human brain tissue stained for STIM2 using ab223033 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-STIM2 antibody (ab223033) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : Mouse heart tissue lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 69,84,94 kDa
Observed band size: 69,84 kDa why is the actual band size different from the predicted?
ab223033 has not yet been referenced specifically in any publications.