Key features and details
- Rabbit polyclonal to STIM2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-STIM2 antibody
See all STIM2 primary antibodies
DescriptionRabbit polyclonal to STIM2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human STIM2 aa 600-650. The exact sequence is proprietary. (NP_065911.3).
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241510 was affinity purified using an epitope specific to STIM2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241510 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 84 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionFunctions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
Tissue specificityExpressed in all tissues and tumor cell lines examined.
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain.
Phosphorylated predominantly on Ser residues.
Cellular localizationEndoplasmic reticulum membrane. Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration.
- Information by UniProt
- FLJ39527 antibody
- KIAA1482 antibody
- STIM 2 antibody
All lanes : Anti-STIM2 antibody (ab241510) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 84 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
STIM2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241510 used for IP at 6 µg per reaction. Western blot was perfomed on the immunoprecipitate using another anti-STIM2 antibody at 1 µg/ml.
Lane 1: ab241510 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab241510 has not yet been referenced specifically in any publications.