Overview

  • Product name

    Anti-STIM2 antibody [EPR12879] - C-terminal
    See all STIM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12879] to STIM2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human STIM2 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9P246

  • Positive control

    • A673 and BxPC3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12879
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

Target

  • Function

    Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
  • Tissue specificity

    Expressed in all tissues and tumor cell lines examined.
  • Sequence similarities

    Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications

    Glycosylated.
    Phosphorylated predominantly on Ser residues.
  • Cellular localization

    Endoplasmic reticulum membrane. Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ39527 antibody
    • KIAA1482 antibody
    • STIM 2 antibody
    • STIM2 antibody
    • STIM2_HUMAN antibody
    • Stromal interaction molecule 2 antibody
    see all

Images

  • All lanes : Anti-STIM2 antibody [EPR12879] - C-terminal (ab181258) at 1/10000 dilution

    Lane 1 : A673 cell lysate
    Lane 2 : BxPC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated) at 1/1000 dilution

    Predicted band size: 84 kDa

References

This product has been referenced in:

  • Pierro C  et al. Oncogenic KRAS suppresses store-operated Ca2+ entry and ICRAC through ERK pathway-dependent remodelling of STIM expression in colorectal cancer cell lines. Cell Calcium 72:70-80 (2018). WB ; Human . Read more (PubMed: 29748135) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181258.
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