Anti-STING antibody - C-terminal (ab189430)
Key features and details
- Rabbit polyclonal to STING - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-STING antibody - C-terminal
See all STING primary antibodies -
Description
Rabbit polyclonal to STING - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human TMEM173 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q86WV6 -
Positive control
- IHC-P: Human tonsil tissue. WB: Mouse spleen tissue lysate. Flow Cyt (Intra): HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 42 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| Flow Cyt (Intra) |
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 42 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
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Flow Cyt (Intra)
1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the TMEM173 family. -
Post-translational
modificationsPhosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation. -
Cellular localization
Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited. - Information by UniProt
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Database links
- Entrez Gene: 340061 Human
- Entrez Gene: 72512 Mouse
- Omim: 612374 Human
- SwissProt: Q86WV6 Human
- SwissProt: Q3TBT3 Mouse
- Unigene: 379754 Human
- Unigene: 45995 Mouse
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Alternative names
- endoplasmic reticulum IFN stimulator antibody
- Endoplasmic reticulum interferon stimulator antibody
- ERIS antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody - C-terminal (ab189430)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling TMEM173 with ab189430 at 5 μg/ml.
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Western blot - Anti-STING antibody - C-terminal (ab189430)Anti-STING antibody - C-terminal (ab189430) at 1/1000 dilution + Mouse spleen tissue lysate at 35 µg
Predicted band size: 42 kDa -
Flow Cytometry (Intracellular) - Anti-STING antibody - C-terminal (ab189430)Intracellular flow cytometric analysis of HeLa cells labeling TMEM173 with ab189430 at 1/10 dilution (right histogram) compared to negative control cells (left histogram) using FITC-conjugated goat-anti-rabbit secondary antibodies.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab189430 has been referenced in 1 publication.
- Zheng B et al. HBsAg Dampened STING Associated Activation of NK Cells in HBeAg-Negative CHB Patients. Int J Mol Sci 22:N/A (2021). PubMed: 34299262
