RecombinantRabMAb

Recombinant Anti-STING antibody [EPR13130] (ab181125)

Reviews (1) Submit a question References (5)
Western blot - Anti-STING antibody [EPR13130] (ab181125)
  • Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [EPR13130] (ab181125)
  • Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)
  • Western blot - Anti-STING antibody [EPR13130] (ab181125)
  • Western blot - Anti-STING antibody [EPR13130] (ab181125)
  • Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)
  • Anti-STING antibody [EPR13130] (ab181125)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13130] to STING
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-STING antibody [EPR13130]
    See all STING primary antibodies

  • Description

    Rabbit monoclonal [EPR13130] to STING

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • THP1 or HACAT lysate, THP1 or HACAT cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/2000. Detects a band of approximately 37 kDa.
ICC/IF
1/2000.

For unpurified, use 1/50 - 1/100.
Flow Cyt
1/10 - 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 37 kDa.
ICC/IF
1/2000.

For unpurified, use 1/50 - 1/100.
Flow Cyt
1/10 - 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the TMEM173 family.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.

  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt

  • Database links

    • Alternative names

      • endoplasmic reticulum IFN stimulator antibody
      • Endoplasmic reticulum interferon stimulator antibody
      • ERIS antibody
      • FLJ38577 antibody
      • hMITA antibody
      • hSTING antibody
      • Mediator of IRF3 activation antibody
      • MITA antibody
      • Mitochondrial mediator of IRF3 activation antibody
      • MPYS antibody
      • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
      • NET23 antibody
      • Stimulator of interferon genes antibody
      • Stimulator of interferon genes protein antibody
      • STING antibody
      • TM173_HUMAN antibody
      • Tmem173 antibody
      • Transmembrane protein 173 antibody
      see all

    Images

    • Western blot - Anti-STING antibody [EPR13130] (ab181125)
      Western blot - Anti-STING antibody [EPR13130] (ab181125)
      All lanes : Anti-STING antibody [EPR13130] (ab181125) at 1/10000 dilution (purified)

      Lane 1 : THP-1 cell lysate
      Lane 2 : HACAT cell lysate
      Lane 3 : human spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Observed band size: 37 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [EPR13130] (ab181125)
      Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [EPR13130] (ab181125)

      Immunocytochemistry/immunofluorescence analysis of THP-1 (human monocytic leukemia monocyte) cells labelling TMEM173 with ab181125 at 10 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
      Confocal image showing cytoplasmic staining in THP-1 cells.

    • Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)
      Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)

      Overlay histogram showing THP-1 cells fixed in 4% PFA and stained with purified ab181125 at a dilution of 1 in 20 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

      Alexa Fluor® 488 (ab198950) and R-PE (ab208874) conjugated versions are available for this clone.

    • Western blot - Anti-STING antibody [EPR13130] (ab181125)
      Western blot - Anti-STING antibody [EPR13130] (ab181125)
      Anti-STING antibody [EPR13130] (ab181125) at 1/2000 dilution (purified) + HEK293 cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Observed band size: 37 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-STING antibody [EPR13130] (ab181125)
      Western blot - Anti-STING antibody [EPR13130] (ab181125)
      All lanes : Anti-STING antibody [EPR13130] (ab181125) at 1/2000 dilution (unpurified)

      Lane 1 : THP1 lysate
      Lane 2 : HACAT lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    • Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)
      Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)

      Flow cytometry analysis of THP1 cells using unpurified ab181125 at a 1/10 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

    • Anti-STING antibody [EPR13130] (ab181125)
      Anti-STING antibody [EPR13130] (ab181125)

    References (5)

    Publishing research using ab181125? Please let us know so that we can cite the reference in this datasheet.

    ab181125 has been referenced in 5 publications.

    • Zheng Z  et al. Irradiation induces cancer lung metastasis through activation of the cGAS-STING-CCL5 pathway in mesenchymal stromal cells. Cell Death Dis 11:326 (2020). PubMed: 32382015
    • Uhlorn BL  et al. Attenuation of cGAS/STING activity during mitosis. Life Sci Alliance 3:N/A (2020). PubMed: 32661021
    • Wu S  et al. HER2 recruits AKT1 to disrupt STING signalling and suppress antiviral defence and antitumour immunity. Nat Cell Biol 21:1027-1040 (2019). PubMed: 31332347
    • Li J  et al. Porcine circovirus type 2 inhibits inter-ß expression by targeting Karyopherin alpha-3 in PK-15 cells. Virology 520:75-82 (2018). WB . PubMed: 29793076
    • Hare DN  et al. Membrane Perturbation-Associated Ca2+ Signaling and Incoming Genome Sensing Are Required for the Host Response to Low-Level Enveloped Virus Particle Entry. J Virol 90:3018-27 (2015). IF ; Human . PubMed: 26719279

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-TMEM173 antibody [EPR13130]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    HEK293
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 10°C
    Read More

    Dr. Robert Kupp

    Verified customer

    Submitted Oct 19 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com