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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Western blot - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
  • Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP339] to STING - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-STING antibody [SP339] - BSA and Azide free
    See all STING primary antibodies

  • Description

    Rabbit monoclonal [SP339] to STING - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig

  • Immunogen

    Synthetic peptide within Human TMEM173 aa 150-350 (C terminal). The exact sequence is proprietary.
    Database link: Q86WV6

  • Positive control

    • WB: Wild-type THP-1 cell lysate. IHC-P: Human tonsil, colon, colon carcinoma, thymus, lymph node, lung, HK lymphoma, lung adenocarcinoma, pancreatic adenocarcinoma, lung squamous cell carcinoma and skin squamous cell carcinoma tissues. Flow Cyt (intra): THP-1 cells.

  • General notes

    ab238796 is the carrier-free version of ab227705.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

Primary incubation for 30 minutes at 4°C.
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.

Primary incubation for 1 hour at room temperature.
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

Primary incubation for 30 minutes at 4°C.
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.

Primary incubation for 1 hour at room temperature.
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

Target

  • Function

    Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the TMEM173 family.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.

  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt

  • Database links

    • Alternative names

      • endoplasmic reticulum IFN stimulator antibody
      • Endoplasmic reticulum interferon stimulator antibody
      • ERIS antibody
      • FLJ38577 antibody
      • hMITA antibody
      • hSTING antibody
      • Mediator of IRF3 activation antibody
      • MITA antibody
      • Mitochondrial mediator of IRF3 activation antibody
      • MPYS antibody
      • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
      • NET23 antibody
      • Stimulator of interferon genes antibody
      • Stimulator of interferon genes protein antibody
      • STING antibody
      • TM173_HUMAN antibody
      • Tmem173 antibody
      • Transmembrane protein 173 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab227705 for 10 mins at room temperature. This image was generated using ab227705, the same clone, but with a different buffer formulation.

    • Western blot - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Western blot - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      All lanes : Anti-STING antibody [SP339] (ab227705) at 1/400 dilution

      Lane 1 : Wild-type THP-1 cell lysate
      Lane 2 : TMEM173 knockout THP-1 cell lysate
      Lane 3 : Human Tonsil tissue lysate
      Lane 4 : Human Thymus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?



      This data was developed using the same antibody clone in a different buffer formulation (ab227705).

      Lanes 1 - 4: Merged signal (red and green). Green - ab227705 observed at 37 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

      ab227705 was shown to react with TMEM173 in wild-type THP-1 cells in Western blot with loss of signal observed in TMEM173 knockout cell line ab270493 (TMEM173 knockout cell lysate ab270516). Wild-type THP-1 and TMEM173 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab227705 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab227705 for 10 mins at room temperature. This image was generated using ab227705, the same clone, but with a different buffer formulation.

    • Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Intracellular Flow Cytometry analysis of THP-1 (human acute monocytic leukemia) labeling TMEM173 with purified ab227705 at 1/20 dilution (12.5µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Intracellular flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227705 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227705).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

      Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227705).

       

    • Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
      Anti-STING antibody [SP339] - BSA and Azide free (ab238796)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab238796? Please let us know so that we can cite the reference in this datasheet.

    ab238796 has been referenced in 1 publication.

    • Chevrier S  et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med 2:100166 (2021). PubMed: 33521697

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com