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Rabbit monoclonal [EPR6605] to STIP1
Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human STIP1 (UniProt P31948).
WB: Jurkat, HeLa, BxPC3, HepG2, and SK OV 3 cell lysates.
IHC-P: Human ovarian carcinoma tissue.
Flow Cyt: HeLa cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
1/10 - 1/100.
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/50 - 1/100.
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
Contains 2 STI1 domains.
Contains 9 TPR repeats.
The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
Information by UniProt
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Western blot - Anti-STIP1 antibody [EPR6605] (ab126724)
All lanes : Anti-STIP1 antibody [EPR6605] (ab126724) at 1/10000 dilution Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate Lane 3 : BxPC3 cell lysate Lane 4 : HepG2 cell lysate Lane 5 : SK OV 3 cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1 antibody [EPR6605] (ab126724)
ab126724, at a dilution of 1/250, staining STIP1 in paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.
Flow Cytometry - Anti-STIP1 antibody [EPR6605] (ab126724)
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1 (red) with ab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor
® 488) ( ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG ( ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
Western blot - Anti-STIP1 antibody [EPR6605] (ab126724) This image is courtesy of an anonymous Abreview
Anti-STIP1 antibody [EPR6605] (ab126724) at 1/1000 dilution + Mouse brain whole tissue lysate at 150 µg
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"