Overview

  • Product name

  • Description

    Rabbit polyclonal to STIP1/STI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human STIP1/STI1 (internal sequence). The exact sequence is proprietary.
    Database link: STIP1

  • Positive control

    • WB: Jurkat, Raji, K562 and THP-1 whole cell lysates. IHC-P: BT43 xenograft tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as STIP1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 63 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
  • Sequence similarities

    Contains 2 STI1 domains.
    Contains 9 TPR repeats.
  • Domain

    The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis secretory sperm binding protein Li 94n antibody
    • HEL S 94n antibody
    • Hop antibody
    • Hsc70/Hsp90 organizing protein antibody
    • Hsc70/Hsp90-organizing protein antibody
    • IEF SSP 3521 antibody
    • NY REN 11 antigen antibody
    • P60 antibody
    • Renal carcinoma antigen NY-REN-11 antibody
    • STI1 antibody
    • STI1L antibody
    • STIP1 antibody
    • STIP1_HUMAN antibody
    • Stress induced phosphoprotein 1 antibody
    • Stress-induced-phosphoprotein 1 antibody
    • Transformation sensitive protein IEF SSP 3521 antibody
    • Transformation-sensitive protein IEF SSP 3521 antibody
    see all

Images

  • All lanes : Anti-STIP1/STI1 antibody (ab228711) at 1/10000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 63 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded BT483 xenograft tissue stained for STIP1/STI1 using ab228711 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for STIP1/STI1 (green) using ab228711 at 1/500 dilution in ICC/IF.

    Alpha-tubulin filaments were labeled with an anti-Alpha-tubulin antibody at 1/2000 dilution (red).

References

ab228711 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228711.
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