Anti-STIP1/STI1 antibody (ab233196)
Key features and details
- Rabbit polyclonal to STIP1/STI1
- Suitable for: IHC-P, WB
- Reacts with: Cow, Human
- Isotype: IgG
Overview
-
Product name
Anti-STIP1/STI1 antibody
See all STIP1/STI1 primary antibodies -
Description
Rabbit polyclonal to STIP1/STI1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey, Chinese hamster -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human STIP1/STI1 aa 1-200. Two N-terminal tags. (Expressed in E.coli).
Database link: P31948 -
Positive control
- IHC-P: Human cerebrum, liver, kidney, stomach, prostate cancer and colorectal cancer tissue. WB: Recombinant human STIP1/STI1 protein; Human lung lysate; Jurkat cell lysate; Cow testis and spleen lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 63 kDa.
|
Notes |
---|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 63 kDa. |
Target
-
Function
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). -
Sequence similarities
Contains 2 STI1 domains.
Contains 9 TPR repeats. -
Domain
The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 617109 Cow
- Entrez Gene: 10963 Human
- Entrez Gene: 20867 Mouse
- Entrez Gene: 192277 Rat
- Omim: 605063 Human
- SwissProt: O54981 Chinese hamster
- SwissProt: Q3ZBZ8 Cow
- SwissProt: Q4R8N7 Cynomolgus monkey
see all -
Alternative names
- Epididymis secretory sperm binding protein Li 94n antibody
- HEL S 94n antibody
- Hop antibody
see all
Images
-
Anti-STIP1/STI1 antibody (ab233196) at 2 µg/ml + Recombinant human STIP1/STI1 protein
Predicted band size: 63 kDa -
All lanes : Anti-STIP1/STI1 antibody (ab233196) at 1 µg
Lane 1 : Human lung lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : Cow testis lysate
Lane 4 : Cow spleen lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 63 kDa -
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for STIP1/STI1 using ab233196 at 20 µg/mL in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for STIP1/STI1 using ab233196 at 20 µg/mL in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human stomach tissue stained for STIP1/STI1 using ab233196 at 20 µg/mL in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human kidney tissue stained for STIP1/STI1 using ab233196 at 20 µg/mL in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human liver tissue stained for STIP1/STI1 using ab233196 at 20 µg/mL in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for STIP1/STI1 using ab233196 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab233196 has not yet been referenced specifically in any publications.