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    stip1sti1-antibody-epr6605-ab126724.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

  • Datasheet
  • SDS
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Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Flow Cytometry - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
  • Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6605] to STIP1/STI1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human STIP1/STI1 protein (ab92357)

View more associated products

Overview

  • Product name

    Anti-STIP1/STI1 antibody [EPR6605]
    See all STIP1/STI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6605] to STIP1/STI1
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human STIP1/STI1 aa 50-150.
    Database link: P31948

  • Positive control

    • WB: HAP1, HEK-293, Jurkat, HeLa, BxPC3, HepG2, and SK OV 3 cell lysates, human, mouse and rat brain lysate. IHC-P: Human ovarian carcinoma, testis, thyroid cancer tissue. Flow Cyt: HeLa cells.
  • General notes

     This product was previously labelled as STIP1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6605
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones

Associated products

  • Alternative Versions

    • Anti-STIP1/STI1 antibody [EPR6605] - BSA and Azide free (ab238963)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Hep G2 cytoplasmic extract lysate (ab14659)
    • Hep G2 nuclear extract lysate (ab14660)
    • Jurkat cytoplasmic extract lysate (ab14842)
  • Recombinant Protein

    • Recombinant Human STIP1/STI1 protein (ab92357)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Mouse
Human
Application Abreviews Notes
WB (6)
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/250- 1/500

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

Flow Cyt
1/20.

For unpurified use at 1/20

Notes
WB
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/250- 1/500

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

Flow Cyt
1/20.

For unpurified use at 1/20

Target

  • Function

    Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
  • Sequence similarities

    Contains 2 STI1 domains.
    Contains 9 TPR repeats.
  • Domain

    The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P31948 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10963 Human
    • Entrez Gene: 20867 Mouse
    • Entrez Gene: 192277 Rat
    • Omim: 605063 Human
    • SwissProt: P31948 Human
    • SwissProt: Q60864 Mouse
    • SwissProt: O35814 Rat
    • Unigene: 337295 Human
    • Unigene: 258633 Mouse
    • Unigene: 22614 Rat
    see all
  • Alternative names

    • Epididymis secretory sperm binding protein Li 94n antibody
    • HEL S 94n antibody
    • Hop antibody
    • Hsc70/Hsp90 organizing protein antibody
    • Hsc70/Hsp90-organizing protein antibody
    • IEF SSP 3521 antibody
    • NY REN 11 antigen antibody
    • P60 antibody
    • Renal carcinoma antigen NY-REN-11 antibody
    • STI1 antibody
    • STI1L antibody
    • STIP1 antibody
    • STIP1_HUMAN antibody
    • Stress induced phosphoprotein 1 antibody
    • Stress-induced-phosphoprotein 1 antibody
    • Transformation sensitive protein IEF SSP 3521 antibody
    • Transformation-sensitive protein IEF SSP 3521 antibody
    see all

Images

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : STIP1 knockout HAP1 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab126724 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab126724 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126724 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified) + Human brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : BxPC3 cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : SK OV 3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Unpurified ab126724, at a dilution of 1/250, staining STIP1/STI1 in paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Flow Cytometry - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1/STI1 (red) with purified ab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)This image is courtesy of an anonymous Abreview
    Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/1000 dilution (Unpurified) + Mouse brain whole tissue lysate at 150 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa


    Exposure time: 5 minutes

    See Abreview

  • Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
    Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab126724? Please let us know so that we can cite the reference in this datasheet.

ab126724 has been referenced in 4 publications.

  • Cui J  et al. Proteomic Identification of Pathways Responsible for the Estradiol Therapeutic Window in AD Animal Models. Front Cell Neurosci 13:437 (2019). PubMed: 31680862
  • Guo X  et al. STIP1 Regulates Proliferation and Migration of Lung Adenocarcinoma Through JAK2/STAT3 Signaling Pathway. Cancer Manag Res 11:10061-10072 (2019). PubMed: 31819639
  • Göllner S  et al. Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nat Med 23:69-78 (2017). PubMed: 27941792
  • He Y  et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). PubMed: 27959342

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain cortex)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 21 2015

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (CNS)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 14 2014

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
150 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Apr 16 2014

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Cerebral Cortex)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
Cerebral Cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Oct 30 2013

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Oct 23 2013

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293)
Gel Running Conditions
Reduced Denaturing (10% Acrylamide)
Loading amount
10 µg
Specification
HEK-293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 20 2013

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