Overview

  • Product name

    Anti-STIP1/STI1 antibody [EPR6605]
    See all STIP1/STI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6605] to STIP1/STI1
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human STIP1/STI1 aa 50-150.
    Database link: P31948

  • Positive control

    • WB: Jurkat, HeLa, BxPC3, HepG2, and SK OV 3 cell lysates, human, mouse and rat brain lysate. IHC-P: Human ovarian carcinoma, testis, thyroid cancer tissue. Flow Cyt: HeLa cells.
  • General notes

     This product was previously labelled as STIP1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/250- 1/500

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

Flow Cyt 1/20.

For unpurified use at 1/20

Target

  • Function

    Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
  • Sequence similarities

    Contains 2 STI1 domains.
    Contains 9 TPR repeats.
  • Domain

    The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis secretory sperm binding protein Li 94n antibody
    • HEL S 94n antibody
    • Hop antibody
    • Hsc70/Hsp90 organizing protein antibody
    • Hsc70/Hsp90-organizing protein antibody
    • IEF SSP 3521 antibody
    • NY REN 11 antigen antibody
    • P60 antibody
    • Renal carcinoma antigen NY-REN-11 antibody
    • STI1 antibody
    • STI1L antibody
    • STIP1 antibody
    • STIP1_HUMAN antibody
    • Stress induced phosphoprotein 1 antibody
    • Stress-induced-phosphoprotein 1 antibody
    • Transformation sensitive protein IEF SSP 3521 antibody
    • Transformation-sensitive protein IEF SSP 3521 antibody
    see all

Images

  • All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified) + Human brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : BxPC3 cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : SK OV 3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 63 kDa

  • Unpurified ab126724, at a dilution of 1/250, staining STIP1/STI1 in paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry.

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1/STI1 (red) with purified ab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/1000 dilution (Unpurified) + Mouse brain whole tissue lysate at 150 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa


    Exposure time: 5 minutes

    See Abreview

References

This product has been referenced in:

  • He Y  et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). Read more (PubMed: 27959342) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Brain cortex)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 21 2015

Application
Western blot
Sample
Human Tissue lysate - whole (CNS)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 14 2014

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
150 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 16 2014

Application
Western blot
Sample
Rat Tissue lysate - whole (Cerebral Cortex)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
Cerebral Cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 30 2013

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 23 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293)
Gel Running Conditions
Reduced Denaturing (10% Acrylamide)
Loading amount
10 µg
Specification
HEK-293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 20 2013

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