Recombinant Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6605] to STIP1/STI1
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-STIP1/STI1 antibody [EPR6605]
See all STIP1/STI1 primary antibodies -
Description
Rabbit monoclonal [EPR6605] to STIP1/STI1 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested Applications & Species
Application Species Flow Cyt HumanIHC-P HumanWB MouseHuman -
Immunogen
corresponding to Human STIP1/STI1 aa 50-150.
Database link: P31948 -
Positive control
- WB: HAP1, HEK-293, Jurkat, HeLa, BxPC3, HepG2, and SK OV 3 cell lysates, human, mouse and rat brain lysate. IHC-P: Human ovarian carcinoma, testis, thyroid cancer tissue. Flow Cyt: HeLa cells.
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General notes
This product was previously labelled as STIP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6605 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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IHC-P |
Human
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WB |
Mouse
Human
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Application | Abreviews | Notes |
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WB | (6) |
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/250- 1/500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
|
Flow Cyt |
1/20.
For unpurified use at 1/20 |
Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/250- 1/500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Flow Cyt
1/20. For unpurified use at 1/20 |
Target
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Function
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). -
Sequence similarities
Contains 2 STI1 domains.
Contains 9 TPR repeats. -
Domain
The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10963 Human
- Entrez Gene: 20867 Mouse
- Entrez Gene: 192277 Rat
- Omim: 605063 Human
- SwissProt: P31948 Human
- SwissProt: Q60864 Mouse
- SwissProt: O35814 Rat
- Unigene: 337295 Human
see all -
Alternative names
- Epididymis secretory sperm binding protein Li 94n antibody
- HEL S 94n antibody
- Hop antibody
see all
Images
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All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : STIP1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDaLanes 1 - 4: Merged signal (red and green). Green - ab126724 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab126724 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126724 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified)
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : SK OV 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
Unpurified ab126724, at a dilution of 1/250, staining STIP1/STI1 in paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1/STI1 (red) with purified ab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)This image is courtesy of an anonymous AbreviewAnti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/1000 dilution (Unpurified) + Mouse brain whole tissue lysate at 150 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 5 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab126724 has been referenced in 4 publications.
- Cui J et al. Proteomic Identification of Pathways Responsible for the Estradiol Therapeutic Window in AD Animal Models. Front Cell Neurosci 13:437 (2019). PubMed: 31680862
- Guo X et al. STIP1 Regulates Proliferation and Migration of Lung Adenocarcinoma Through JAK2/STAT3 Signaling Pathway. Cancer Manag Res 11:10061-10072 (2019). PubMed: 31819639
- Göllner S et al. Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nat Med 23:69-78 (2017). PubMed: 27941792
- He Y et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). PubMed: 27959342