Name: Anti-STIP1/STI1 antibody [EPR6605]
Brand: RabMAb
SKU: ab126724
Rating: 5 (6 reviews)

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Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6605] to STIP1/STI1
Suitable for: Flow Cyt (Intra), WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-STIP1/STI1 antibody [EPR6605]
See all STIP1/STI1 primary antibodies
Description

Rabbit monoclonal [EPR6605] to STIP1/STI1
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HAP1, HEK-293, Jurkat, HeLa, BxPC3, HepG2, and SK OV 3 cell lysates, human, mouse and rat brain lysate. IHC-P: Human ovarian carcinoma, testis, thyroid cancer tissue. Flow Cyt (intra): HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6605
Isotype

IgG
Research areas

Alternative Versions
- Anti-STIP1/STI1 antibody [EPR6605] - BSA and Azide free (ab238963)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human STIP1/STI1 protein (ab92357)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/20. For unpurified use at1/20
WB	(6)	1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/250- 1/500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols.

Notes
Flow Cyt (Intra) 1/20. For unpurified use at1/20
WB 1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/250- 1/500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols.

Function

Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
Sequence similarities

Contains 2 STI1 domains.
Contains 9 TPR repeats.
Domain

The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
Cellular localization

Cytoplasm. Nucleus.
Target information above from: UniProt accession P31948 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10963 Human
- Entrez Gene: 20867 Mouse
- Entrez Gene: 192277 Rat
- Omim: 605063 Human
- SwissProt: P31948 Human
- SwissProt: Q60864 Mouse
- SwissProt: O35814 Rat
- Unigene: 337295 Human
- Unigene: 258633 Mouse
- Unigene: 22614 Rat
see all
Alternative names
- Epididymis secretory sperm binding protein Li 94n antibody
- HEL S 94n antibody
- Hop antibody
- Hsc70/Hsp90 organizing protein antibody
- Hsc70/Hsp90-organizing protein antibody
- IEF SSP 3521 antibody
- NY REN 11 antigen antibody
- P60 antibody
- Renal carcinoma antigen NY-REN-11 antibody
- STI1 antibody
- STI1L antibody
- STIP1 antibody
- STIP1_HUMAN antibody
- Stress induced phosphoprotein 1 antibody
- Stress-induced-phosphoprotein 1 antibody
- Transformation sensitive protein IEF SSP 3521 antibody
- Transformation-sensitive protein IEF SSP 3521 antibody
see all

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : STIP1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 63 kDa
Observed band size: 63 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab126724 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab126724 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab126724 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified)

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 63 kDa
Observed band size: 63 kDa
Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified) + Human brain lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 63 kDa
Observed band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : SK OV 3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Unpurified ab126724, at a dilution of 1/250, staining STIP1/STI1 in paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1/STI1 (red) with purifiedab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)This image is courtesy of an anonymous Abreview

Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/1000 dilution (Unpurified) + Mouse brain whole tissue lysate at 150 µg

Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 63 kDa
Observed band size: 63 kDa

Exposure time: 5 minutes
See Abreview
Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab126724? Please let us know so that we can cite the reference in this datasheet.

ab126724 has been referenced in 5 publications.

Beckley SJ et al. STIP1/HOP Regulates the Actin Cytoskeleton through Interactions with Actin and Changes in Actin-Binding Proteins Cofilin and Profilin. Int J Mol Sci 21:N/A (2020). PubMed: 32365744
Cui J et al. Proteomic Identification of Pathways Responsible for the Estradiol Therapeutic Window in AD Animal Models. Front Cell Neurosci 13:437 (2019). PubMed: 31680862
Guo X et al. STIP1 Regulates Proliferation and Migration of Lung Adenocarcinoma Through JAK2/STAT3 Signaling Pathway. Cancer Manag Res 11:10061-10072 (2019). PubMed: 31819639
Göllner S et al. Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nat Med 23:69-78 (2017). PubMed: 27941792
He Y et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). PubMed: 27959342

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1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Human Tissue lysate - whole (Brain cortex)

Gel Running Conditions

Reduced Denaturing (12)

Loading amount

50 µg

Specification

Brain cortex

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 21 2015

Application

Western blot

Sample

Human Tissue lysate - whole (CNS)

Gel Running Conditions

Reduced Denaturing (12)

Loading amount

30 µg

Specification

CNS

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 14 2014

Application

Western blot

Sample

Mouse Tissue lysate - whole (Brain)

Gel Running Conditions

Reduced Denaturing (15)

Loading amount

150 µg

Specification

Brain

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 16 2014

Application

Western blot

Sample

Rat Tissue lysate - whole (Cerebral Cortex)

Gel Running Conditions

Reduced Denaturing (12)

Loading amount

40 µg

Specification

Cerebral Cortex

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 30 2013

Application

Western blot

Sample

Mouse Tissue lysate - whole (brain)

Gel Running Conditions

Reduced Denaturing (12)

Loading amount

40 µg

Specification

brain

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 23 2013

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK-293)

Gel Running Conditions

Reduced Denaturing (10% Acrylamide)

Loading amount

10 µg

Specification

HEK-293

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 20 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (5)

Customer reviews and Q&As

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]

Western blot abreview for Anti-STIP1/STI1 antibody [EPR6605]