Overview

  • Product name

    Anti-STIP1/STI1 antibody [EPR6605] (HRP)
    See all STIP1/STI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6605] to STIP1/STI1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human STIP1/STI1.
    Database link: P31948

  • Positive control

    • WB: Jurkat, HeLa and BxPC3 whole cell lysates. IHC-P: FFPE human normal testis tissue sections.
  • General notes

     

     

     This product was previously labelled as STIP1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab202919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • Function

    Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
  • Sequence similarities

    Contains 2 STI1 domains.
    Contains 9 TPR repeats.
  • Domain

    The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis secretory sperm binding protein Li 94n antibody
    • HEL S 94n antibody
    • Hop antibody
    • Hsc70/Hsp90 organizing protein antibody
    • Hsc70/Hsp90-organizing protein antibody
    • IEF SSP 3521 antibody
    • NY REN 11 antigen antibody
    • P60 antibody
    • Renal carcinoma antigen NY-REN-11 antibody
    • STI1 antibody
    • STI1L antibody
    • STIP1 antibody
    • STIP1_HUMAN antibody
    • Stress induced phosphoprotein 1 antibody
    • Stress-induced-phosphoprotein 1 antibody
    • Transformation sensitive protein IEF SSP 3521 antibody
    • Transformation-sensitive protein IEF SSP 3521 antibody
    see all

Images

  • IHC image of STIP1/STI1 staining in a section of formalin-fixed paraffin-embedded human normal testis*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab202919, 1/200 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-STIP1/STI1 antibody [EPR6605] (HRP) (ab202919) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : BxPC3 (Human pancreas adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202919 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab202919 has not yet been referenced specifically in any publications.

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