Overview

  • Product name

    Anti-STIP1/STI1 antibody [EPR6606]
    See all STIP1/STI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6606] to STIP1/STI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human STIP1/STI1 aa 450-550. The exact sequence is proprietary.

  • Positive control

    • Human ovarian carcinoma tissue; Jurkat, HeLa, BxPC3, and HepG2 whole cell lysate (ab7900).
  • General notes

     

     

     This product was previously labelled as STIP1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.)
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
    • Sequence similarities

      Contains 2 STI1 domains.
      Contains 9 TPR repeats.
    • Domain

      The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Epididymis secretory sperm binding protein Li 94n antibody
      • HEL S 94n antibody
      • Hop antibody
      • Hsc70/Hsp90 organizing protein antibody
      • Hsc70/Hsp90-organizing protein antibody
      • IEF SSP 3521 antibody
      • NY REN 11 antigen antibody
      • P60 antibody
      • Renal carcinoma antigen NY-REN-11 antibody
      • STI1 antibody
      • STI1L antibody
      • STIP1 antibody
      • STIP1_HUMAN antibody
      • Stress induced phosphoprotein 1 antibody
      • Stress-induced-phosphoprotein 1 antibody
      • Transformation sensitive protein IEF SSP 3521 antibody
      • Transformation-sensitive protein IEF SSP 3521 antibody
      see all

    Images

    • All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : BxPC3 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 63 kDa

    • ab126753, at 1/50 dilution, staining STIP1/STI1 in paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.

    • ab126753 immunoprecipitating STIP1/STI1 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at a dilution of 1/30.

      Blocking and diution buffer: 5% NFDM/TBST.



      All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution

      Lane 1 : IP using ab126753 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

      Observed band size: 63 kDa
      why is the actual band size different from the predicted?


      Exposure time: 2 minutes

    References

    This product has been referenced in:

    • M Fouad E  et al. The Expression of FOXE-1 and STIP-1 in Papillary Thyroid Carcinoma and Their Relationship with Patient Prognosis. Iran J Pathol 13:256-271 (2018). Read more (PubMed: 30697297) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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