Recombinant Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
![Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-414428-anti-stip1sti1-antibody-epr6606-bsa-and-azide-free-western-blot-hap1-wt-hap1-stip1-knockout-a431-hek293.jpg "Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6606] to STIP1/STI1 - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free
See all STIP1/STI1 primary antibodies -
Description
Rabbit monoclonal [EPR6606] to STIP1/STI1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, A431, HEK293, BxPC-3, Jurkat, HeLa, and HepG2 cell lysates. IHC-P: Human ovarian carcinoma tissue.
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General notes
ab248167 is the carrier-free version of ab126753 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248167 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as STIP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6606 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
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| IP |
Human
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| WB |
Human
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| All applications |
Mouse
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| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. |
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
Target
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Function
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). -
Sequence similarities
Contains 2 STI1 domains.
Contains 9 TPR repeats. -
Domain
The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10963 Human
- Entrez Gene: 20867 Mouse
- Entrez Gene: 192277 Rat
- Omim: 605063 Human
- SwissProt: P31948 Human
- SwissProt: Q60864 Mouse
- SwissProt: O35814 Rat
- Unigene: 337295 Human
see all -
Alternative names
- Epididymis secretory sperm binding protein Li 94n antibody
- HEL S 94n antibody
- Hop antibody
see all
Images
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Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : STIP1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab126753).
Lanes 1 - 4: Merged signal (red and green). Green - ab126753 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab126753 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126753 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-2-anti-stip1-sti1-antibody-epr6606-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)This data was developed using ab126753, the same antibody clone in a different buffer formulation. ab126753, at 1/50 dilution, staining STIP1/STI1 in paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-405424-anti-stip1-sti1-antibody-epr6606-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDaThis data was developed using ab126753, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-399685-anti-stip1-sti1-antibody-epr6606-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)This data was developed using ab126753, the same antibody clone in a different buffer formulation.
ab126753 immunoprecipitating STIP1/STI1 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at a dilution of 1/30.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : IP using ab126753 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
![Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)](https://www.abcam.com/ps/products/248/ab248167/Images/ab248167-5-benefits-of-recombinant-antibodies.png)
Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free (ab248167)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab248167 has not yet been referenced specifically in any publications.