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Rabbit monoclonal [EPR10306] to STK25
ICC/IF more details Unsuitable for:
Flow Cyt or IP
Synthetic peptide, corresponding to residues in Human STK25 (UniProt O00506).
HeLa, HepG2, A431, fetal brain, Human testis and K562 lysates; Human heart tissue; HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
1/50 - 1/100.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.
Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm. Golgi apparatus. Localizes to the Golgi apparatus.
Information by UniProt
Serine/threonine kinase 25 antibody
Serine/threonine kinase 25 (STE20 homolog, yeast) antibody
Western blot - Anti-STK25 antibody [EPR10306] (ab157188)
All lanes : Anti-STK25 antibody [EPR10306] (ab157188) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : HepG2 cell lysate Lane 3 : A431 cell lysate Lane 4 : Human fetal brain lysate Lane 5 : Human testis lysate Lane 6 : K562 cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK25 antibody [EPR10306] (ab157188)
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling STK25 with ab157188 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-STK25 antibody [EPR10306] (ab157188)
Immunofluorescent analysis of HepG2 cells labeling STK25 with ab157188 at 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"