Recombinant Anti-STK25 antibody [EPR10306] (ab157188)
![Western blot - Anti-STK25 antibody [EPR10306] (ab157188)](https://www.abcam.com/ps/products/157/ab157188/Images/ab157188-164337-anti-stk25-antibody-epr10306-western-blot.jpg "Western blot - Anti-STK25 antibody [EPR10306] (ab157188)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10306] to STK25
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STK25 antibody [EPR10306]
See all STK25 primary antibodies -
Description
Rabbit monoclonal [EPR10306] to STK25 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human STK25 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- HeLa, HepG2, A431, fetal brain, Human testis and K562 lysates; Human heart tissue; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10306 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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Function
Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. -
Tissue specificity
Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Golgi apparatus. Localizes to the Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 10494 Human
- Omim: 602255 Human
- SwissProt: O00506 Human
- Unigene: 516807 Human
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Alternative names
- C86992 antibody
- Serine/threonine kinase 25 antibody
- Serine/threonine kinase 25 (STE20 homolog, yeast) antibody
see all
Images
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Western blot - Anti-STK25 antibody [EPR10306] (ab157188)All lanes : Anti-STK25 antibody [EPR10306] (ab157188) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human fetal brain lysate
Lane 5 : Human testis lysate
Lane 6 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK25 antibody [EPR10306] (ab157188)](https://www.abcam.com/ps/products/157/ab157188/Images/ab157188-164336-anti-stk25-antibody-epr10306-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK25 antibody [EPR10306] (ab157188)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling STK25 with ab157188 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-STK25 antibody [EPR10306] (ab157188)](https://www.abcam.com/ps/products/157/ab157188/Images/ab157188-164335-anti-stk25-antibody-epr10306-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-STK25 antibody [EPR10306] (ab157188)Immunofluorescent analysis of HepG2 cells labeling STK25 with ab157188 at 1/100 dilution. -
![Anti-STK25 antibody [EPR10306] (ab157188)](https://www.abcam.com/ps/products/157/ab157188/Images/ab157188-4-benefits-of-recombinant-antibodies.png)
Anti-STK25 antibody [EPR10306] (ab157188)
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab157188 has been referenced in 2 publications.
- Bae SJ et al. STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism. Elife 9:N/A (2020). PubMed: 32292165
- Lim S et al. Identification of the kinase STK25 as an upstream activator of LATS signaling. Nat Commun 10:1547 (2019). PubMed: 30948712
