Recombinant
RabMAb

Anti-STK25 antibody [EPR10306] (ab157188)

Overview

  • Product name
    Anti-STK25 antibody [EPR10306]
    See all STK25 primary antibodies
  • Description
    Rabbit monoclonal [EPR10306] to STK25
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human STK25 (UniProt O00506).

  • Positive control
    • HeLa, HepG2, A431, fetal brain, Human testis and K562 lysates; Human heart tissue; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.
    • Tissue specificity
      Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Golgi apparatus. Localizes to the Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C86992 antibody
      • Serine/threonine kinase 25 antibody
      • Serine/threonine kinase 25 (STE20 homolog, yeast) antibody
      • Serine/threonine kinase 25 (STE20 homolog, yeast), isoform CRA_a antibody
      • Serine/threonine kinase 25 (yeast) antibody
      • Serine/threonine protein kinase 25 antibody
      • Serine/threonine-protein kinase 25 antibody
      • SOK 1 antibody
      • SOK-1 antibody
      • SOK1 antibody
      • Ste20 like kinase antibody
      • Ste20 yeast homolog antibody
      • Ste20-like antibody
      • STE20-like kinase antibody
      • Ste20/oxidant stress response kinase 1 antibody
      • Sterile 20 (oxidant stress response kinase 1) antibody
      • Sterile 20/oxidant stress-response kinase 1 antibody
      • STK25 antibody
      • STK25_HUMAN antibody
      • Yeast Sps1/Ste20 related kinase 1 antibody
      • YSK 1 antibody
      • YSK1 antibody
      see all

    Images

    • All lanes : Anti-STK25 antibody [EPR10306] (ab157188) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Human fetal brain lysate
      Lane 5 : Human testis lysate
      Lane 6 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 48 kDa

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling STK25 with ab157188 at 1/50 dilution.
    • Immunofluorescent analysis of HepG2 cells labeling STK25 with ab157188 at 1/100 dilution.

    References

    ab157188 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7- breast cancer cell line)
    Gel Running Conditions
    Reduced Denaturing (Bolt® 10% Bis-Tris Plus)
    Loading amount
    30 µg
    Treatment
    +/- siRNA
    Specification
    MCF7- breast cancer cell line
    Blocking step
    Li-Cor 50% TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: rtm°C
    Username

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    Verified customer

    Submitted Dec 23 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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