Product nameAnti-STK3 / MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody
DescriptionRabbit polyclonal to STK3 / MST-2 (phospho T180) + MSP/MST1 (phospho T183)
Specificityab79199 detects endogenous levels of STK3/MST1 only when phosphorylated at threonine 183 (MST1) and/or threonine T180 (STK3).
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide (phospho T183). Synthetic phosphopeptide derived from human STK3/MST1 around the phosphorylation site of threonine 183 (R-N-TP-V-I).
- WB: Extracts from 293 cells treated with H2O2 (100uM, 15mins). IHC-P: Human liver carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab79199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/50 - 1/100.|
Cellular localizationSTK3: Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm. MST1: Secreted.
All lanes : Anti-STK3 / MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody (ab79199)
Lane 1 : Extracts from 293 cells, treated
with H2O2 (100uM, 15mins)
Lane 2 : Extracts from 293 cells, treated
with H2O2 (100uM, 15mins) with immunising phosphopeptide
Predicted band size: 56 kDa
Observed band size: 56 kDa
ab79199, at 1/50 dilution, staining STK3 (phospho T180) + MSP/MST1 (phospho T183) in human liver carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
This product has been referenced in:
- Wu W et al. Early protective role of MST1 knockdown in response to experimental diabetic nephropathy. Am J Transl Res 8:1397-411 (2016). Read more (PubMed: 27186267) »
- Dixit D et al. Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent inhibition of glucose metabolism. Cell Death Dis 5:e1212 (2014). WB ; Human . Read more (PubMed: 24810048) »