Anti-STK3 / MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody (ab79199)

Overview

  • Product name

    Anti-STK3 / MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody
  • Description

    Rabbit polyclonal to STK3 / MST-2 (phospho T180) + MSP/MST1 (phospho T183)
  • Host species

    Rabbit
  • Specificity

    ab79199 detects endogenous levels of STK3/MST1 only when phosphorylated at threonine 183 (MST1) and/or threonine T180 (STK3).
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (phospho T183). Synthetic phosphopeptide derived from human STK3/MST1 around the phosphorylation site of threonine 183 (R-N-TP-V-I).

  • Positive control

    • WB: Extracts from 293 cells treated with H2O2 (100uM, 15mins). IHC-P: Human liver carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

Images

  • All lanes : Anti-STK3 / MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody (ab79199)

    Lane 1 : Extracts from 293 cells, treated
    with H2O2 (100uM, 15mins)
    Lane 2 : Extracts from 293 cells, treated
    with H2O2 (100uM, 15mins) with immunising phosphopeptide

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • ab79199, at 1/50 dilution, staining STK3 (phospho T180) + MSP/MST1 (phospho T183) in human liver carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

References

This product has been referenced in:

  • Wu W  et al. Early protective role of MST1 knockdown in response to experimental diabetic nephropathy. Am J Transl Res 8:1397-411 (2016). Read more (PubMed: 27186267) »
  • Dixit D  et al. Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent inhibition of glucose metabolism. Cell Death Dis 5:e1212 (2014). WB ; Human . Read more (PubMed: 24810048) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (5-15%)
Loading amount
20 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Taha Rehmani

Verified customer

Submitted Oct 04 2018

Question

Catalog number(s): 79199
Product(s): Rabbit polyclonal to MST1/MST2 (phospho T183)
-----------------------------------

Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. No
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A.

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I requested protocol advice
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes

Q. Did the replacement product work successfully?
A.I have not tried it yet
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Completely unsatisfied
Q. Could we have done anything to better resolve the problem?
A.better troubleshooting and advice
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.

Read More
Answer

Thank you for your participation in Abcam's post complaint survey.

Could you please let me know if the replacement product worked well for you?

Please remember that our guarantee is good only for 6 months.
We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

I had sent the free of charge replacement out of goodwill although the antibody you had used had been ordered about 2 years ago.


If using antibodies in untested applications or species, please let us know in advance. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.



Please let me know If there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits).

Read More

Question
Answer

Thank you for your reply.

Could you please let me know the institution you are working at?
I have located an order for ab79199, but am not sure if this is the correct one. For ab63474, the PO or order number was not included in your email.

Please let me know this information and also if you like to replace, credit or refund the antibody ab63474. If you prefer a replacement, I would also need to know which antibody I shall replace it with.

I look forward to hear back from you.

Read More

Answer

Thank you for your reply.
I am very sorry to hear that the tips did not help to improve the results.

Please let me know your order or PO number. If ab63474 was purchased within the last 6 months, I can replace, credit or refund the antibody. As for ab79199, since mouse is not tested, I can send you a testing discount code which gives you a free antibody upon submission of an Abreview with your negative data.

I am very sorry about the negative outcome for both antibodies, but hopefully we can resolve this case one way or another to your satisfaction.

I look forward to your reply. Please let me know which of the options you would prefer.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your reply.

Since ab79199 has not been tried on mouse samples, we do not guarantee it for this species. The guarantee applies for tested species and applications only.

I would like to suggest trying other antigen retrieval methods, e.g. Tris/EDTA or enzymatic as this can help with obtaining a signal. This is the only step I can think of that might prevent you from obtaining a signal.

Please let me know if trying this does improve your results. If not, please let me know as well includingyour order or PO number.

I hope this information is helpful to you and I look forward to hear back.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.
I am sorry to hear that the antibodies are not working as expected.

Your protocol looks fine to me. I wanted to ask you a few more questions:

1) Both antibodies detect a phospho-site. Under the experimental conditions you are using is the threonine 183 phosphorylated?
2) ab79199 has not been tried on mouse samples, thus we do not know if and how well the antibody works on this species.
3) Have you tried other antigen retrieval methods, e.g. Tris/EDTA or enzymatic?
4) Do the detection systems work well with other primary antibodies?
5) Have you tried a positive control, such as human brain sections or human liver carcinoma sections?

Please also let me know your order number.

I look forward to hear back from you and assist you further.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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