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    stk31-antibody-ab69678.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-STK31 antibody (ab69678)

  • Datasheet
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Western blot - Anti-STK31 antibody (ab69678)

    Key features and details

    • Mouse polyclonal to STK31
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human STK31 protein (ab127094)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-STK31 antibody
      See all STK31 primary antibodies
    • Description

      Mouse polyclonal to STK31
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Full length human STK31 protein (AAH59374.1).

    • Positive control

      • STK31 transfected 293T lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.4
      Constituent: 2.68% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Cancer
      • Tumor biomarkers
      • Tumor antigens

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)
    • Recombinant Protein

      • Recombinant Human STK31 protein (ab127094)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab69678 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (1)
    1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).

    Target

    • Tissue specificity

      Testis specific.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
      Contains 1 Tudor domain.
    • Target information above from: UniProt accession Q9BXU1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 56164 Human
      • Omim: 605790 Human
      • SwissProt: Q9BXU1 Human
      • Unigene: 309767 Human
      • Alternative names

        • Serine/threonine kinase 31 antibody
        • Serine/threonine protein kinase 31 antibody
        • Serine/threonine protein kinase NYD SPK antibody
        • Serine/threonine-protein kinase 31 antibody
        • Serine/threonine-protein kinase NYD-SPK antibody
        • SgK396 antibody
        • STK 31 antibody
        • Stk31 antibody
        • STK31_HUMAN antibody
        • Sugen kinase 396 antibody
        • TDRD8 antibody
        • Testis tissue sperm-binding protein Li 78m antibody
        • Tudor domain containing 8 antibody
        see all

      Images

      • Western blot - Anti-STK31 antibody (ab69678)
        Western blot - Anti-STK31 antibody (ab69678)
        All lanes : Anti-STK31 antibody (ab69678) at 1/500 dilution

        Lane 1 : STK31 transfected 293T lysate
        Lane 2 : Non-transfected 293T lysate

        Lysates/proteins at 25 µg per lane.

        Secondary
        All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

        Predicted band size: 116 kDa
        Observed band size: 130 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab69678? Please let us know so that we can cite the reference in this datasheet.

      ab69678 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-STK31 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (ES cells)
      Loading amount
      20 µg
      Specification
      ES cells
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 20 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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