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    stk32a-antibody-n-terminal-ab170394.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-STK32A antibody - N-terminal (ab170394)

  • Datasheet
  • SDS
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Western blot - Anti-STK32A antibody - N-terminal (ab170394)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK32A antibody - N-terminal (ab170394)

Key features and details

  • Rabbit polyclonal to STK32A - N-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-STK32A antibody - N-terminal
  • Description

    Rabbit polyclonal to STK32A - N-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human STK32A aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8WU08

  • Positive control

    • Human bladder carcinoma tissue; mouse spleen tissue lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab29293)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Application Abreviews Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/100 - 1/500. Predicted molecular weight: 46 kDa.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/100 - 1/500. Predicted molecular weight: 46 kDa.

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q8WU08 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 202374 Human
    • Entrez Gene: 269019 Mouse
    • SwissProt: Q8WU08 Human
    • SwissProt: Q8BGW6 Mouse
    • Unigene: 585069 Human
    • Unigene: 150401 Mouse
    • Alternative names

      • A930015B13Rik antibody
      • MGC22688 antibody
      • Serine/threonine kinase 32A antibody
      • Serine/threonine protein kinase 32A antibody
      • Serine/threonine-protein kinase 32A antibody
      • ST32A_HUMAN antibody
      • STK 32A antibody
      • Stk32a antibody
      • YANK 1 antibody
      • YANK1 antibody
      • Yet another novel kinase 1 antibody
      see all

    Images

    • Western blot - Anti-STK32A antibody - N-terminal (ab170394)
      Western blot - Anti-STK32A antibody - N-terminal (ab170394)
      Anti-STK32A antibody - N-terminal (ab170394) at 1/100 dilution + mouse spleen tissue lysate at 35 µg

      Predicted band size: 46 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK32A antibody - N-terminal (ab170394)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK32A antibody - N-terminal (ab170394)

      Immunohistochemical analysis of formalin fixed and paraffin embedded Human bladder carcinoma labeling STK32A with ab170394 at 1/50 dlution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab170394? Please let us know so that we can cite the reference in this datasheet.

    ab170394 has not yet been referenced specifically in any publications.

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