Key features and details
- Rabbit polyclonal to STK33
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-STK33 antibody
See all STK33 primary antibodies
DescriptionRabbit polyclonal to STK33
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment, corresponding to a region within amino acids 55-469 of Human STK33 (Uniprot ID: Q9BYT3).
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human Cal27 xenograft tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 58 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityHighly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- Hypothetical protein FLJ35932 antibody
- Serine threonine kinase 33 antibody
- Serine/threonine kinase 33 antibody
Anti-STK33 antibody (ab155056) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 58 kDa
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling STK33 with ab155056 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labeling STK33 with ab155056 at 1/100 dilution.
ab155056 has not yet been referenced specifically in any publications.