Overview

  • Product name

    Anti-STK33 antibody [EPR15343]
    See all STK33 primary antibodies
  • Description

    Rabbit monoclonal [EPR15343] to STK33
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human STK33 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BYT3

  • Positive control

    • WB; Jurkat and HEK293 whole cell lysates, Human testis lysate, HUman fetal kidney and spleen lysates. IHC-P; Human testis and Human fetal cardiac muscle tissue. ICC/IF; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15343
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 58, 50 kDa (predicted molecular weight: 58, 50 kDa).
ICC/IF 1/1000.

Target

  • Tissue specificity

    Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Hypothetical protein FLJ35932 antibody
    • Serine threonine kinase 33 antibody
    • Serine/threonine kinase 33 antibody
    • Serine/threonine protein kinase 33 antibody
    • Serine/threonine-protein kinase 33 antibody
    • STK 33 antibody
    • Stk33 antibody
    • STK33_HUMAN antibody
    see all

Images

  • All lanes : Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

     

    Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively

     

  • Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58, 50 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

     

    Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively

  • All lanes : Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds;
    Lane 2: 3 minutes

     

     

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and cytoplasmic staining on human testis tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human fetal cardiac tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and cytoplasmic staining on human fetal cardiac muscle tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STK33 with ab206296 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and nuclear staining on Jurkat cell line is observed.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)  (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab206296 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2. -  anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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