Key features and details
- Mouse monoclonal [3067C3a] to STK3/MST-2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-STK3/MST-2 antibody [3067C3a]
See all STK3/MST-2 primary antibodies
DescriptionMouse monoclonal [3067C3a] to STK3/MST-2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human STK3/MST-2.
- WB: HeLa, HAP1 and NIH3T3 cell lysates; Recombinant human STK3/MST-2.
Previously labelled as STK3.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab71960 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab71960 in the following tested applications.
|WB||Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 56 kDa).|
FunctionStress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
Tissue specificityExpressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain.
Cellular localizationCytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm.
- Information by UniProt
- 0610042I06Rik antibody
- EC 220.127.116.11 antibody
- FLJ90748 antibody
All lanes : Anti-STK3/MST-2 antibody [3067C3a] (ab71960) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : STK3 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Lanes 1- 2: Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab71960 was shown to react with STK3/MST-2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265082 (knockout cell lysate ab257714) was used. Wild-type HeLa and STK3 knockout HeLa cell lysates were subjected to SDS-PAGE. ab71960 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: STK3/MST-2 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: 293T cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab71960 was shown to recognize STK3/MST-2 when STK3/MST-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and STK3/MST-2 knockout samples were subjected to SDS-PAGE. ab71960 and ab181602 (loading control to STK3/MST-2) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Anti-STK3/MST-2 antibody [3067C3a] (ab71960) + immunising recombinant protein
Predicted band size: 56 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab71960 has not yet been referenced specifically in any publications.